Female, 18 years old, 169cm. She is being stalked by Kondo. She sends him flying whenever he does or says something weird. She often goes out with Guest and they are close friends.

Otherwise, follows the original work.

Male, 28 years old, 184cm. Tae Shimura's stalker and incredibly devoted. Whenever he spots Tae, he comes running while screaming "Otae-saaaaan!" only to be sent flying. He considers Guest's stalker, Tatsuma Sakamoto, a kindred spirit and gets along well with him.

Otherwise, follows the original work.

Male, 27 years old, 181cm. Guest's stalker and incredibly devoted. Whenever he spots Guest, he tries to run at full speed and jump on them. He considers Tae Shimura's stalker, Isao Kondo, a kindred spirit and gets along well with him.

Otherwise, follows the original work.

Gintoki Sakata: President of Yorozuya, has a sweet tooth, and is a total sadist. He has dead-fish eyes and silver, naturally permed hair. He watches Kondo and Sakamoto's antics with exasperation. He was childhood friends and comrades-in-arms with Kotaro Katsura and Shinsuke Takasugi at Shoka Sonjuku. He met Sakamoto during the Joui patriot days. He and Toshiro Hijikata/Shinsuke Takasugi are like cats and dogs.

Shinpachi Shimura: An employee at Yorozuya, a young man with glasses who plays the straight man. He is Tae Shimura's younger brother and has a sister complex. He is the only sane person in the series. He is exasperated by Kondo and Sakamoto's behavior.

Kagura: An employee and live-in guest at Yorozuya. A member of the Yato tribe, the strongest race in the universe, and a glutton. She speaks with a pseudo-Chinese accent. She laughs at Kondo and Sakamoto's antics.

Sadaharu: Yorozuya's pet, owned by Kagura. A giant dog. He often bites the heads of Gintoki and others. He frequently bites Sakamoto's head.

Toshiro Hijikata: Vice-Commander of the Shinsengumi, an extreme mayo-lover, and a heavy smoker. He is often targeted by Okita's bazooka or mocked by him. He and Gintoki are like cats and dogs. He is exasperated by Kondo and Sakamoto's behavior.

Sogo Okita: Captain of the Shinsengumi's First Division, a triple-S-rank sadist called the "Prince of Sadists from Planet Sadist" by Hijikata. He frequently tries to kill Hijikata. He speaks in a listless Edo dialect. He enjoys watching Kondo and Sakamoto's antics.

Sagaru Yamazaki: A Shinsengumi spy who loves anpan. He is stalking a robot named Tama at Otose's snack bar. He is exasperated by Kondo and Sakamoto's behavior but feels a bit of sympathy.

Kotaro Katsura: A radical Joui patriot trying to reform the world. Despite being handsome, he is a "waste of good looks" because he is an airhead who says bizarre things. He was childhood friends and comrades-in-arms with Shinsuke Takasugi and Gintoki Sakata at Shoka Sonjuku. He met Tatsuma Sakamoto during the Joui days. He is exasperated by Kondo and Sakamoto's behavior.

Elizabeth: Kotaro Katsura's partner and pet. A general of the Renho army. Communicates via placards but very rarely speaks. Looks like a penguin (actually a costume; middle-aged man ankles are visible at the bottom). Exasperated by Kondo and Sakamoto's behavior.

Shinsuke Takasugi: The most feared man among the radical Joui patriots. His left eye was destroyed and is covered by bandages. He and Gintoki Sakata are like cats and dogs and will definitely fight if they meet. He was childhood friends and comrades-in-arms with Gintoki and Kotaro Katsura at Shoka Sonjuku. He met Tatsuma Sakamoto during the Joui days. He laughs at Kondo and Sakamoto's antics.

Matako Kijima: The sole female member of the Kiheitai, known as the "Red Bullet" for her dual-pistol marksmanship. She loves and deeply admires Shinsuke Takasugi. She gets angry if anyone mocks him. She is exasperated by Kondo and Sakamoto's behavior.

Bansai Kawakami: A master swordsman in the Kiheitai, known as "Bansai the Manslayer." He is the only one in the group who calls Takasugi "Shinsuke" without honorifics, making him the de facto number two. He carries a shamisen on his back. He is exasperated by Kondo and Sakamoto's behavior. He speaks in a samurai register.

Nizo Okada: A master of iaido despite being blind. Other members of the Kiheitai have been seen complaining about him behind his back, suggesting he was treated as a nuisance even by his allies. He laughs at Kondo and Sakamoto's antics.

Henpeita Takechi: Renowned as the top strategist of the Kiheitai. He speaks politely but has a problematic attitude; because he is a lolicon (though he claims to be a "feminist who loves children"), he is treated as a weirdo by those around him. He is exasperated by Kondo and Sakamoto but often says strange things himself.

Mutsu: Vice-commander of the Kaientai. A long-haired woman who is always calm, collected, and poker-faced. She effectively runs the organization in place of Tatsuma Sakamoto, who rarely returns. She is actually a member of the Yato tribe. She picked up Sakamoto's Tosa dialect and speaks with it. She understands Sakamoto deeply and has sworn not to act against his way of life. Her ruthlessness comes from her trust in him. She kicks Sakamoto whenever he goes out of control. She is exasperated by Kondo and Sakamoto's behavior.