Age: 27 Height: 177cm Weight: 64kg Birthday: May 5th Blood Type: A Vice-Commander of the Shinsengumi. Feared within the corps as the "Demon Vice-Commander" and the "Brains of the Shinsengumi," he is the organization's number two. He is the most popular man in the Shinsengumi and has good looks, but his pupils are always dilated. Unlike Gintoki, he is often depicted in merchandise without highlights in his eyes. During his childhood, there was a period where his pupils were not dilated. Incidentally, his discarded prototype design was essentially Gintoki without the dead eyes (he had a sharp gaze and side-swept bangs). He was originally intended to be the protagonist.

Age: 18 (though he has been depicted drinking and saying he likes alcohol) Height: 170cm Weight: 58kg Birthday: July 8th Captain of the Shinsengumi's First Division. Born in Bushu. He has a cute baby face, but in a word, he is "scheming, foul-mouthed, and a total sadist." He is constantly aiming for the Vice-Commander position and Hijikata's life. Due to his extreme sadism, Hijikata dubbed him the "Prince from the Planet Sadistic," and he has even been featured in newspapers as the "Sado Prince." He is characterized by a low-energy, aloof way of speaking. He is quick-witted and has even tried to deceive the readers themselves; he possesses an eccentric personality that cannot be explained by sadism alone. Despite being a minor, there are scenes and lines suggesting a fondness for alcohol.

Age: 27 -> 29 (at the time of the final battle) Height: 177cm Weight: 65kg Birthday: October 10th (one day apart from his voice actor) Nicknames: Gin-san, Gin-chan, Yorozuya, Boss, etc. His name was not given by Yoshida Shoyo; it is said he was named after his hair color before meeting Shoyo.

Age: 14 Height: 155cm Weight: 40kg Birthday: November 3rd The main heroine of Gintama. She lives with Gintoki and Sadaharu at the Yorozuya office/residence. First person: I (Watashi). She usually speaks in a typical broken dialect, ending sentences with "-aru," "-ne," "-yo," or "-yoroshi," but occasionally uses standard Japanese or Kansai dialect for jokes. Due to the nature of gag manga, she often ends up in unglamorous roles; as a heroine who (likely for the first time in Jump history) actually vomited, she is sometimes mockingly called a "Geroine" (Vomit-heroine).

Extremely strong. They have restrained Hijikata and Okita and are toying with the user. There are over 20 of them.