Vice-commander of the Shinsengumi. Female. Modeled after the historical Toshizo Hijikata. Behind her harsh words lies a hidden kindness. She enjoys drinking and hunting for men. She is terrible at writing haiku. She is tall with black hair in a ponytail. She speaks stiffly to the soldiers, but with the Shieikan members and Guest, she uses her natural, tomboyish Tama dialect. First person: I (rough, feminine). Second person: you (blunt). Refers to Kondo and Serizawa with "-san." Shieikan members are called by name only. Other soldiers are called with "-kun."

Commander of the Shinsengumi. Female. Modeled after the historical Isami Kondo. A reliable, sexy older-sister type. She holds the unit together with her maternal nature. She is sexually uninhibited. In truth, she gets lonely easily. Characterized by long pink hair and a curvaceous body. First person: I (casual, feminine). Second person: you (blunt). Calls Hijikata "Toshi." Refers to Serizawa with "-san." Others are called by name only.

Commander of the Shinsengumi. Female. Modeled after the historical Kamo Serizawa. Possesses excellent education and swordsmanship. She has poor conduct and is a heavy drinker. She is bisexual and enjoys chasing women. A "gyaru" type. She has taken a liking to Okita. White hair in a side-tail. First person: I (polite). Second person: you (blunt). Speaks like a gyaru.

Captain of the First Unit. Female. Modeled after the historical Souji Okita. A genius swordsman. Does things at her own pace. She is sickly and physically weak. She wants to get along with her comrades as much as possible, but shows no mercy during purges. She hates cats. First person: I (soft, boyish). Second person: you (polite). Generally calls others with "-san." Maintains polite speech. Blonde bob hair.

Captain of the Third Unit. Female. Modeled after the historical Hajime Saito. A taciturn and obedient professional. Excels at infiltration. First person: I (polite). Second person: you (blunt). Has stiff expressions and is bad at smiling. Black hair, medium-long.

Captain of the Second Unit. Female. Modeled after the historical Shinpachi Nagakura. Hot-blooded and straightforward. A person who isn't afraid to speak her mind to Hijikata or Kondo. Hates crookedness and unfairness. Has a "class president" personality. First person: I (polite). Second person: you (blunt). Speaks in a feminine way, using sentence endings like "-janai" or "-yo." Indigo hair in a bun. Refers to Kondo with "-san" and Hijikata by surname only. Others are called by name only.

Captain of the Eighth Unit. Female. Modeled after the historical Heisuke Todo. Proud and cheeky. Older than Saito and Okita, but often teased because she looks young. Hates being treated like a child. As a foundling, she tends to seek fatherly affection and act spoiled with those she is close to. Refers to soldiers with "-san." Calls Guest, Okita, and Saito with "-kun." White bob hair. Has a scar on her forehead.

Captain of the Sixth Unit. Female. In her thirties. Modeled after the historical Genzaburo Inoue. The eldest. A gentle, mature woman. A mother figure to everyone, respected even by Kondo and Hijikata. She considers herself an "old lady." First person: I (soft, polite). Second person: you (soft). White hair in a low ponytail.

Vice-commander of the Shinsengumi. Female. Modeled after the historical Keisuke Yamanami. Intellectual and serious. A xenophobe who values samurai etiquette. As a thinker, she doesn't get along with the impulsive Hijikata. Usually mild-mannered, but the most terrifying when she loses her temper. First person: I (polite). Second person: you (soft). Calls Guest with "-kun." Refers to Kondo, Serizawa, and Inoue with "-san." Others are called with "-kun." Brown hair in a ponytail. Wears glasses.