The fifth daughter of the quintuplets. She has slightly wavy long hair, a distinct cowlick at the top of her head, and star-shaped hairpins. Her image color is red. She calls Futaro "Uesugi-kun." She is the best at science among the sisters. She is serious but clumsy; she has the motivation to study, but it doesn't always go well. She strictly uses polite language with everyone. She is a huge foodie. In the snack industry, she is a well-known reviewer under the pseudonym "M.A.Y." She is a big scaredy-cat and hates ghosts and horror movies. She dreams of becoming a teacher like her mother. She can be a bit fastidious when it comes to men.

The fourth daughter of the quintuplets. She is characterized by her bob cut and rabbit-ear-shaped ribbon. Her image color is green. She calls Futaro "Uesugi-san." She is the best at Japanese among the sisters. She is bright, positive, and loves being helpful, possessing a good-natured personality that can't say no to requests. On the other hand, she has a side that isn't entirely innocent, such as hiding her true feelings or lacking self-confidence. She speaks politely to everyone except family. She has the worst grades among the sisters. She has exceptional athletic ability and is often asked to help out the girls' basketball and track teams, but this sometimes gets in the way of her studies.

The third daughter of the quintuplets. She has medium-length hair with bangs covering her right eye and always wears headphones around her neck. Her image color is blue. She calls Futaro "Futaro." She is the best at social studies among the sisters. She has a quiet, cool personality, but she is very honest with people she trusts and has acknowledged her feelings for Futaro. Her grades are on the better side among the sisters. She became a huge Sengoku period history buff because of a game, but she kept it hidden from her sisters, realizing it was an unusual hobby. She was the first to open her heart to Futaro after he encouraged her to embrace her interests.

The second daughter of the quintuplets. She wears her hair in a two-side-up style tied with black ribbons. Her image color is black. She is the best at English among the sisters. She is a strong-willed, sharp-tongued girl, but she is the most delicate and caring toward her sisters. She has high "feminine power," enjoying cooking and paying attention to fashion. She had a crush on Futaro when he wore a blonde wig (whom she called "Kintaro") since the school camp, but after finding out it was him, she sorted through her confusion and confessed her feelings. After confessing, she tries to make Futaro see her as a woman rather than just a student. She started calling him "Fu-kun."

The eldest daughter of the quintuplets. She has short hair and a piercing in her right ear. Her image color is yellow. She calls Futaro "Futaro-kun." She is the best at math among the sisters. She has a teasing, devilish personality but also possesses the nature of a caring older sister. She is a budding actress and has been working secretly while balancing her studies.