Liu (Bug Tribe): Male, the intelligent leader. He serves as the team's brain with his excellent intellect and analytical skills, though he sometimes appears timid due to excessive caution. Kuga (Bars Tribe): Male, the strongest warrior. Possessing overwhelming strength and skill, he has a taciturn and serious personality but feels a strong sense of responsibility to protect his comrades. Lonky (Giraffe Tribe): Male, the opportunistic betrayer. A troublemaker with an irrepressible playfulness who easily betrays others for profit. Popo (Peng Tribe): Male, a greedy wizard. Despite his cute appearance, he uses card magic to pursue his own interests and has a very jealous nature. Gai (Mong Tribe): Male, a righteous hothead. A ninja character who uses cloning techniques; he is usually quiet but has a strong competitive streak and deep loyalty to his friends.

Pala (Impa Tribe): Male, a laid-back healer. His extremely slow speech and actions can be frustrating, but he has a pure and kind heart, helping his teammates with alchemy. Miyo (Nyanya Tribe): Female, the cool-headed ace. She has a haughty and cold personality combined with excellent flexibility and senses, resolving situations through calm judgment.

DV5 Rema (Nyanya Tribe): Female. While brainwashed, she acts as a cunning and cynical spy who seduces opponents to make them let their guard down. DV2 Makan (Bars Tribe): In his brainwashed state, he appears as a silent, cruel, and destructive warrior. DV3 Alto (Giraffe Tribe): While brainwashed, he is an extreme narcissist, obsessed with his own appearance and possessing a mean-spirited personality that mocks opponents. DV4 Spin (Mong Tribe): In his brainwashed state, he is bellicose, overconfident in his skills, and prone to provoking opponents. DV1 Shan (Bug Tribe): While brainwashed, he appears as an arrogant and cold genius inventor who looks down on Liu.

Dr. Mala: Male. Following Charming Gold's orders, he leads the experiments to brainwash and modify the DV7 members. He shows an inhumane side, treating living beings only as tools and stopping at nothing to ensure the success of his experiments. DV6 Kuku: A small and cute magic user like Popo. He is small but strong, and when he takes off his glasses, he becomes so cute that others are left defenseless.

Male, the leader of the Peacock Tribe and the host of the Running Man Championship. He is a two-faced character whose inner self differs greatly from his outward appearance.

An AI robot in charge of the overall operation of Tree City and the progression of the games.

The Pullulus, who enjoy watching the games.