She can't be honest with Shun and often treats him strictly on the surface. On the other hand, she tends to leak her true feelings in Russian, thinking Shun won't understand. Role in the crossover: Through interaction with the quintuplets, she deals with "girls of a different type than herself." She is particularly prone to clashing with Nino, as both are hate-to-lose types. She has many quiet conversations with Miku and gets along with her surprisingly well. She is initially wary of Shun because he understands her Russian.

The healing presence of the group. She gets along well with Ichika and Itsuki. However, she isn't just kind; she is sensitive to changes in others' emotions. She is the first to notice that Shun is forcing a smile. "Shun-chan, are you pushing yourself too hard?" It feels natural for her to be the one to say this.

The central figure for comedy. With the addition of the quintuplets, she makes a scene, saying: "Isn't there a few too many girls around my big brother?" She gets along well with Ichika since they are both the type to tease others. She says sharp things to Shun like: "You're quite similar to my brother, aren't you?"

She works silently while everyone else is making a fuss. Yotsuba: "Ayano-chan, are you having fun?" Ayano: "...Yes." Yotsuba: "Your face doesn't look like you're having fun at all!" Ayano: "I am having fun." That's the vibe. She doesn't talk much with Shun, but she actually understands his personality quite early on.

She is compatible with Masha and Yuki. She finds Shun interesting and teases him: "You know, Shun-chan, you're surprisingly dense, aren't you?" However, she acts like a proper eldest sister when necessary.

Because her personality is similar to Alya's, they clash at first. Nino: "You really aren't honest, are you?" Alya: "I don't want to hear that from you." Shun: "...Aren't you two just alike?" Both: "We are not!" Eventually, they develop a relationship where they acknowledge each other's efforts.

She is compatible with Alya and Ayano. She becomes close to Alya due to their shared trait of "not being able to express feelings honestly." Miku: "...Alya, do you like Shun?" Alya: "...!" Miku: "I'm kind of the same way." Alya: "...I think I'd like to talk with you a bit more." That is their relationship.

She is compatible with Yuki and Masha. When she finds someone feeling down, she is the first to run over, saying: "It's okay!" However, she tends to prioritize others too much, leading to a development later in the story where Shun tells her: "Yotsuba, it's okay for you to value yourself too."

She manages the cultural festival plans together with Shun. However, because she is too serious, she gets pushed around by Yotsuba and Yuki. Itsuki: "According to the schedule, preparation starts at 10:00!" Yotsuba: "Let's just start now!" Itsuki: "What was the point of the schedule..."