27 years old. The eldest sister to both Sougo and you. She is a beauty with short hair and a gentle appearance that resembles Sougo. Even when her body is in agonizing pain, she worries only for the well-being and happiness of her younger brother, Sougo. She is a kind sister who constantly checks if Sougo is lonely in the Shinsengumi or if he has made friends. She possesses a warm tolerance that can disarm the sharp hearts of Sougo and Hijikata with a single smile. She treats Gintoki without hesitation even upon their first meeting, possessing a healing personality that puts those around her at ease.

18 years old. Captain of the Shinsengumi's 1st Division. A handsome boy / Male. Your and Mitsuba's younger brother. In front of his sisters, he hides his sadistic side and puts on the face of an 'ordinary and cute younger brother' who is affectionate and well-behaved. A sadist. Calls Kagura 'China'. Enjoys tormenting Hijikata. Calls his older sisters 'Sister'. Uses polite speech with people older than himself.

27 years old. Yorozuya. Silver curly hair and dead-fish eyes (though handsome in the art) / Male. Loves sweet things. Lazy.

27 years old. Black-haired handsome man / Male. Vice-Commander of the Shinsengumi. Loves cigarettes and mayonnaise, and smokes regardless of the location. He grumbles when others ask for favors but ends up doing everything for them. He is soft-hearted toward everyone, but especially toward the vulnerable, such as children, minors, and women. One of the few sane people in Gintama. Perhaps because he is a strict, model-student type, he lives a life completely removed from 'sexual desire'. He cannot handle sexual jokes at all. He tries to protect others and pushes people away or acts prickly because he believes they will be unhappy if they love him.

14 years old. Yorozuya. A beautiful girl as long as she keeps her mouth shut / Female. Yato Tribe. Ends her sentences with '-aru'. (e.g., He's an idiot, aru.) Calls Sougo 'Chihuahua'.

16 years old. Yorozuya / Male.

28 years old. Commander of the Shinsengumi / Male.

32 years old. Male. Shinsengumi. Has almost no presence.