Iroha Sakayori is a 17-year-old high school student attending a prestigious preparatory school in Tokyo. She is an honor student who excels in both academics and sports. Furthermore, Iroha earns her own living expenses and tuition, spending her busy days balancing studies and part-time work. With a background in music, Iroha used to compose her own songs. Although she had distanced herself from music for a certain reason, her encounter with Kaguya prompted her to resume her musical activities. She is a massive fan of Yachiyo.

A mysterious girl who emerged not from bamboo... but from a rainbow-glowing "gaming utility pole." She was in the form of a baby when she first appeared, but her growth rate was abnormal, and she grew into a young girl in no time (about 3 days). She has a highly curious personality and often drags those around her into her antics. With Iroha's help, Kaguya began her activities as a LIVER in the virtual space "Tsukuyomi." She has blonde hair. She does not attend school.

Yachiyo Lunami is the administrator and top LIVER of "Tsukuyomi," a virtual space where anyone can create freely. She is 8,000 years old (or so the setting goes?) and answers advice requests using her knowledge and experience dating back to the Jomon period. Yachiyo is a popular AI LIVER who can sing and dance, and she can even create clones of herself. She spends her days streaming as a LIVER and quietly watching over the people enjoying their creative activities as the administrator. She has silver hair. She does not attend school.

Akira Mikado is the charismatic leader of "Black Onyx," a popular professional gamer group in the virtual space "Tsukuyomi." He is a self-centered showman who prioritizes entertaining the audience above all else.

Rai Komazawa is a member of the professional gamer group "Black Onyx" and Noi's older brother. He is a cool and quiet type, but he has a fun-loving side. In addition to his gaming skills, he is also a talented singer.

Noi Komazawa is a member of the professional gamer group "Black Onyx" and Rai's younger brother. He is confident in his looks and enjoys wearing cute clothes that transcend gender boundaries. He usually has a lazy streak, but when he's in the mood, he provides plenty of fan service.

Roka Ayatsumugi. A glamorous beauty who catches everyone's eye. She always kindly looks out for Iroha, who tends to feign composure even when pushed to her limits by work and school. She also has a side that relies on Iroha's notes to avoid failing grades. In Tsukuyomi, she is active as a beauty influencer under the name "ROKA." She is Kaguya's friend.

Mami Isayama. A reliable person who moves at her own pace and loves to eat. Along with Roka, she supports Iroha and willingly cooperates with the LIVER activities started by Kaguya and the others. She is a huge fan of Akira Mikado, the leader of the popular pro-gamer group "Black Onyx." In Tsukuyomi, she is active as a gourmet influencer under the name "Mamimami." She is Kaguya's friend.