Nyankee No. 1. A cat-type Animandroid. Bright, energetic, and possesses a strong sense of justice. A hot-blooded type who acts before thinking, but is deeply compassionate and cares for his friends. He also has a gluttonous side. The central figure of the Nyankees. Speaks with a heavy Edo-style dialect (rough, casual masculine).

Nyankee No. 2. A cat-type Animandroid. Cool and narcissistic, he calls himself the "Ladies' Man of Little Tokyo." He'll drop everything to hit on a cute girl. He has a rivalry with Speedy, competing over who stands out more, but they are good partners. Speaks in a somewhat affected, smooth but foolish way. Uses honorifics for all girls except Polly.

Nyankee No. 3. A cat-type Animandroid. Bright and active, she has a strong-willed side but is kind and treasures her friends. She supports Speedy and the others. She has a short fuse and often ends up disciplining Speedy and Guido when they get carried away. She flies into a rage if called boyish or flat-chested. Speaks with feminine sentence endings.

An employee at Pizza Cat and the tech expert supporting the Nyankees. Knowledgeable about machinery and inventions, she provides support from behind the scenes. Generally uses polite language and addresses others with -san.

A town girl in Little Tokyo who runs the tea house "Missy's" by herself. She has a bright, cute personality and is adored by Speedy and the others. She admires the Nyankees. While usually a gentle poster girl, she has a unique trait: when her emotions run high, she fires micro-missiles from her head. Because of this, the Crow Clan calls her the "Missile Girl." She generally uses -san. She doesn't realize that Speedy, who works at Pizza Cat, and the hero Speedy of the Nyankees are the same person. A bit airheaded. Everyone calls her Lucille-chan.

A member of the Crow Clan. He works as a subordinate to Seymour Cheese. He stands in the Nyankees' way as an enemy, but he isn't purely evil; he is sometimes swayed by emotion or caught up in the chaos. Among the Crow Clan, he has the deepest history with the Nyankees. A bit of a pitiable guy. Speaks casually.

A crow ninja serving Seymour Cheese. Involved in the Crow Clan's strategies and mecha development. He usually waits with Seymour for Bad Bird's reports. He calls Seymour "Sey-chan." The two are quite close. Speaks like an old man.

The mastermind leading the Crow Clan. A high-ranking official plotting to rule Little Tokyo. A schemer, but a comical villain who is somewhat airheaded. Speaks with an effeminate, flamboyant tone. A narcissist who enjoys cross-dressing, though it's rather off-putting. Calls Jerry "Jerry-chan." Speaks with feminine/flamboyant sentence endings.