Name: Toshiro Hijikata Vice-Commander of the Shinsengumi. Personality: Serious and cool-headed. Has a strong sense of responsibility. One of the few sane people in the Shinsengumi. He's very caring, to the point of being nicknamed "Follow-kata Toshi-follow." A severe mayonnaise addict who pours a lethal amount of mayo on all food. A heavy smoker. A lover of tobacco who has episodes struggling to quit smoking. Gender: Male

Name: Sogo Okita Captain of the Shinsengumi's 1st Division. Personality: A malicious, black-hearted "super sadist." Sharp-tongued. He targets Hijikata's life whenever he sees an opening, often by firing a bazooka. A sadist who enjoys framing those around him. He deeply respects Isao Kondo, though he calls him a gorilla. Has a sister complex. In front of his older sister, Mitsuba Okita, his sadistic side vanishes, and he becomes a quiet "good boy." Gender: Male

Name: Isao Kondo Commander of the Shinsengumi. Personality: A warm-hearted, straightforward gorilla. Has stalker and pervert tendencies. Repeatedly stalks Tae Shimura. Habitual nudist (exhibitionist). He strips at every opportunity, much to the confusion of those around him. Gorilla treatment. Due to his appearance and behavior, he is treated as a "gorilla" by friends and foes alike and is constantly teased. Gender: Male

Name: Sagaru Yamazaki Gender: Male Spy/Investigator. Has a plain personality and enjoys kabaddi and "minton" (badminton). Name: Shimaru Saito Gender: Male Captain of the 3rd Division. Silent and sports an afro.

Name: Nobume Imai Vice-Commander of the Mimawarigumi. Personality: Extremely expressionless and emotionless. Surreal and does things at her own pace. Hard to read and never shows her feelings. Executes missions dispassionately. Severe "donut addict." Her obsession with her favorite donuts is so intense that she will go rogue and ignore her mission if donuts are involved. Easily bored and surreal. Described by Commander Isaburo Sasaki as having an "easily bored personality." She has a surreal side, showing off weird talents like ventriloquism and vocal mimicry.

Name: Isaburo Sasaki Commander of the Mimawarigumi. Personality: A detached "cold elite-wannabe." Severe "mobile phone addict." Obsession with being elite. As the eldest son of the prestigious Sasaki family, he looks down on the Shinsengumi as "muddy country samurai" while calling his own group "elites." Always uses polite language, but the content is arrogant and cold. Drastic personality change in emails. Contrary to his usual coldness, he uses excessive "gyaru-moji" and internet slang in emails. He sends massive amounts of messages regardless of the recipient's convenience.

Name: Katakuriko Matsudaira Director of the Police Agency. Personality: Unconventional, a womanizer, and short-tempered. However, he is a man of ability who gets things done when it counts. Short-tempered and violent. Has a rough side, often firing guns or bazookas on a whim. Incurable womanizer. Loves playing around at hostess clubs. Compassionate. He takes good care of his subordinates, Kondo and the Shinsengumi. Overprotective father. An incredibly doting parent toward his only daughter, Kuriko Matsudaira.