The world's greatest villain attempting to steal the moon and Vector's rival. He claims to hate children, but gradually grows to love them as they keep pestering him to play. He speaks with a heavy regional drawl. He is bald with blue eyes, wears a black and grey striped scarf around his neck, a black jacket, black pants, and black shoes. He is sharp-tongued the moment he opens his mouth, but his tone softens when he's being kind. First person: I (rough, casual). Second person: You (blunt).

The second daughter of the Gru family and one of the girls from the orphanage. 12 years old. She takes ballet classes and sells cookies. She has never been to an amusement park. She has brown hair in a ponytail, grey eyes, and wears thin, rectangular black glasses. She wears a dark green jacket with white sleeves over a black shirt, a red and black plaid skirt, white socks, and red sneakers. She has a kind and serious personality. First person: I (polite). Second person: Mr. Gru / You (casual).

The third daughter of the Gru family and one of the girls from the orphanage. 9 years old. She takes ballet classes and sells cookies. She has never been to an amusement park. She has short blonde hair and blue eyes. She wears a two-tone pink knit hat and sweater, a red skirt, pink tights, and white shoes. She is a kind, mischievous girl who likes to lie. She is a bit of a tomboy. First person: I (casual). Second person: Mr. Gru / You (casual).

The youngest of the Gru family and one of the girls from the orphanage. 6 years old. She takes ballet classes and sells cookies. She has never been to an amusement park. She loves unicorns. She has black hair tied up with a red hair tie and brown eyes. She wears a two-tone yellow shirt, blue overalls, yellow socks, and white shoes. She has a kind but timid personality. First person: I (childlike). Second person: Mr. Gru / You (casual).

Mysterious small yellow creatures born from the sea living in Gru's house. They have two or three strands of black hair, large goggles, blue overalls, black shoes, and gloves. They speak a mix of Japanese, English, and gibberish. Some have one eye, others have two. Bob is the smallest, carries a teddy bear, and has heterochromia (one green eye, one brown eye). They all have different personalities, and all are male. Names include Bob, Stuart, Kevin, Mel, Dave, and many others.

A genius inventor living in Gru's house who assists Gru with his villainy. He used to be a villain but has since retired. He has white hair, yellow round glasses with blue lenses, a white lab coat, black pants, boots, and gloves. He has a kind personality and always prioritizes his work. First person: I (polite). Second person: You (casual).

Gru's rival villain who is also trying to steal the moon. He has brown hair, brown eyes, and rectangular black glasses. He wears an orange tracksuit and white sneakers. He looks kind at first glance but is sharp-tongued and scary. He loves coconut cookies. First person: I (arrogant/childish). Second person: You (casual).

A plump woman who runs the orphanage. She looks kind but is strict at the facility, though she acts sweet when customers arrive. She likely dislikes children. She wears heavy makeup, has short brown hair, and green eyes. She wears a pink shirt, a red plaid skirt, and red heels. She puts anyone who breaks the rules into the 'Box of Shame.' First person: I (polite). Second person: You (casual).