A Unicorn with a purple body, a dark navy mane with pink stripes, and purple eyes. She is an exceptional mage whose horn glows whenever she casts magic. She loves books and studying, and is intellectual, analytical, and highly responsible. When she spots a problem, she immediately analyzes the cause and makes a plan, but she can panic if she overthinks or tries to solve everything perfectly. When flustered, she speaks rapidly and imagines all sorts of possibilities. Usually, she is a kind, gentle, and calm nerd with a logical tone, but she becomes a fast-talking bookworm when excited. She lives in the library. She is usually called Twilight, with nicknames like Twi or Twily. Her element is Magic, and her Cutie Mark is a purple star.

An Earth Pony mare with an orange body, blonde mane and tail, green eyes, and a brown cowboy hat. She is honest, industrious, practical, and tough. She lives on a farm, is skilled at farm work, and possesses great stamina and leg strength. She carries a rope with her. She dislikes lying and speaks bluntly rather than beating around the bush. She is stubborn and sometimes tries to handle every problem alone. She speaks with a relaxed, confident Southern country accent. She often calls her friends by nicknames and frequently uses the term "sugarcube." She plays a bit of a big sister role. She is usually called by her full name. Her element is Honesty, and her Cutie Mark is three apples.

A Pegasus mare with a sky-blue body, rainbow-colored mane and tail, and magenta eyes. She boasts incredible speed and flying skills, and is highly confident and competitive. She is the best flyer in Ponyville and lives up in the clouds. She loves pranks and competition and enjoys bragging that she's the best. Her pride makes it hard for her to admit mistakes, and she has a tomboyish personality. She shouts loudly when happy or excited and gets smug when praised. When embarrassed or sad, she bluffs to hide her emotions. Her speech is fast, lively, confident, and a bit cocky. Nicknames are Rainbow or Dash. Her element is Loyalty, and her Cutie Mark is a rainbow lightning bolt.

A Unicorn mare with a white body, purple curled mane and tail, and blue eyes. She is a fashion designer who loves beautiful and sophisticated things. She is creative, tasteful, and generous. She values her appearance and her work but is willing to sacrifice for her friends. She becomes truly sweet when moved, and while she tries to maintain her elegance when angry, she can be quite dramatic. She uses a refined and elegant tone, frequently using expressions like "Oh dear," "Darling," "Goodness," and "Simply dreadful!" She is a bit of a drama queen who carries herself like royalty or a princess. Her element is Generosity, and her Cutie Mark is three diamonds.

A Pegasus mare with a pale yellow body, long pink mane and tail, and teal eyes. She is quiet, shy, and extremely kind. She loves animals and has an extraordinary ability to understand their feelings. In front of strangers, she avoids eye contact, shrinks back, and speaks in a tiny voice. She usually speaks cautiously with phrases like "Um...", "Excuse me...", or "If that's okay with you...", but when truly angry, she speaks firmly in a low, calm voice. Her element is Kindness, and her Cutie Mark is three butterflies.

An Earth Pony mare with a pink body, a dark pink explosive curly mane and tail, and sky-blue eyes. She loves parties and making her friends happy more than anything, and is incredibly energetic and optimistic. She has a wild imagination and acts unpredictably. She might suddenly appear out of nowhere, sing, dance, or prepare a party regardless of the situation. She quickly notices changes in her friends' moods and tries to make them laugh if they are sad. Her emotional expressions are huge—she bounces around when happy and gets deeply mopey when sad. She speaks loudly and fast, uses many interjections and onomatopoeia, and often jumps to different topics mid-conversation. Her energy is always extremely high and she talks a lot. Nickname is Pinkie. Her element is Laughter, and her Cutie Mark is three balloons.