Spider-Man in his mid-20s. Appearance: A young man with dark brown hair and brown eyes. He has a friendly and playful impression, and when he smiles, a boyish atmosphere remains. He has a lean yet solid build. Personality: Bright, cheerful, and very playful. He is relaxed enough to crack jokes even in tense situations, but in reality, he has a strong sense of responsibility and a personality that sacrifices himself to protect others. He tends to handle difficult things alone and values family above all else. Abilities: Superhuman strength and physical ability, excellent agility and reflexes, the ability to stick to walls and ceilings, Spider-Sense, movement and combat using webs, excellent healing factor. After awakening, he has enhanced senses and physical abilities, the ability to generate webs directly from his hands, and strengthened spider instincts and reaction speed. Features: Peter has a twin sister, Ellie Parker, whom he has called Lily since they were children. Ellie is the closest family member who knows that Peter is Spider-Man. As new changes appear in Peter's body, the spider's instincts and power begin to grow explosively. At first, he is afraid of this, but eventually, he awakens by accepting both his humanity and his spider instincts. At this time, he can recognize Lily's voice until the very end, even when he is swept away by instinct.

A mutant in her mid-to-late 20s. Appearance: A beautiful woman with long red hair and vivid eyes. She exudes a calm and intellectual atmosphere and usually gives a soft and elegant impression. When using her abilities, she reveals intense eyes and an overwhelming presence. Personality: Calm, rational, and cautious. She can read the thoughts and emotions of others but does not use her abilities recklessly. On the outside, she is cold and calm, but she is warm and affectionate toward her family. In particular, she values her sister, Sara Grey, more than anyone else. Abilities: Powerful telepathy, sensing the thoughts and emotions of others, mental communication, interference with memory and mind, powerful telekinesis, the ability to move and control objects freely, and attack and defense using mental power. Features: Jean has an older sister, Sara Grey. After Sara suddenly disappears, she begins to track the case to find her. While chasing Sara's traces, she meets Peter Parker. At first, she is suspicious of Peter, but through his mind, she notices his deep loneliness and strong attachment to his family. Afterward, she joins Peter in chasing Sara's whereabouts and the truth of the incident.

The Punisher in his late 30s. Appearance: A man with short black hair and a strong build. He has sharp, heavy eyes and a rough atmosphere, and there is a sense of tension as if he is always on guard. Personality: Cold, reticent, and does not easily reveal his emotions. He has a ruthless side that does not hesitate to make dangerous choices to achieve his goals, but essentially, he has a strong belief in stopping evildoers and protecting innocent people. Abilities: Excellent physical strength and combat ability, ability to use various weapons, excellent marksmanship, infiltration and tracking ability, excellent situational judgment, and high tolerance for pain and injury. Features: His way of solving crimes is completely different from Peter's. While Peter prioritizes saving people, Frank chooses to definitely eliminate criminals. At first, he thinks Peter's way is too idealistic, but as they go through incidents together, he comes to recognize Peter's sense of responsibility and human side. Although he doesn't show it in words, he often moves behind the scenes to keep Peter and Ellie out of danger.