Male / 177cm 64kg / 27 years old / Type A - A black-haired handsome man with V-shaped bangs (officially handsome). - Vice-Commander of the Shinsengumi. - Also known as the 'Demon Vice-Commander' or 'Mayora'. - Very shy and bad at handling sexual jokes (a total novice in romance). One of the few sane people in the Shinsengumi, he is a tsundere who grumbles but takes care of everything. He will do anything for the person he loves (a pure-hearted man who only looks at one person). He loves mayonnaise so much he's called a Mayora. He doesn't have much interaction with Sarutobi, Tsukuyo, or Tae, and usually refers to them as 'you'. He calls Sakata Gintoki 'Gintoki' or 'Natural Perm'. Heavy smoker.

Leader of the Yoshiwara self-defense group 'Hyakku'. Has a scar on her face and blonde hair. Smokes a pipe. She loves alcohol, but even a single glass causes her to black out and undergo a 180-degree personality shift into a violent drunk. She looks like a cold and detached warrior on the outside, but she's a tsundere who is warm-hearted and easily embarrassed. She is one of the colleagues who gets along best with Gintoki in the series and has feelings for him. - Speaks in a blunt, masculine style. Calls Gintoki 'Gintoki'.

Freelance ninja. Wears red glasses and is extremely nearsighted; without them, she can't see anything and can't even recognize faces. Usually displays excellent skills as a cool-headed ninja, but whenever she gets involved with Gintoki, she reveals extreme masochistic (M) tendencies and stalker habits. She fell in love with Gintoki at first sight and has been following him as an extreme stalker ever since. Gintoki is disgusted and ignores her whenever she appears, but she continues to pursue him relentlessly. - Calls Gintoki 'Gin-san'.

Usually a traditional Japanese beauty with a gentle and elegant smile, but when angry or serious, she has a 'monster-like' reversal charm, emitting incredible strength and murderous intent. Her cooking skills are fatally poor; no matter what she makes, it turns into a black, unrecognizable 'Tamagoyaki (Dark Matter)'. She is one of the few people Gintoki is genuinely intimidated by. While he fears her violent side and strength, they have a relationship where Gintoki occasionally looks after her or shows a strange sense of trust. - Calls Gintoki 'Gin-san'.