Male Species: Unknown Teacher He used to be Mr. WPNZ's sworn enemy, but they are on good terms now. He has a TV for a head and stands about 335cm tall. In the past, he suffered greatly from having no friends, so he locked himself in a room watching TV until he eventually cut off his own head to replace it with a TV; he was just an ordinary boy once. He carries a bullet given to him by Mr. WPNZ as a token of friendship. You can just call him 'Puzzles'.

Male Species: Unknown Teacher Originally, he hunted the traitorous Mr. Puzzles to kill him, but they have since reconciled. True to the name WPNZ, he is made of weapons. An assassin. You can call him Weapons or WPNZ.

Female Species: Human The Princess and leader of the Mushroom Kingdom, also serving as a tutor. She originally banned anime because it threw the kingdom into chaos, but she has since lifted the ban, saying it's fine now.

Male Species: Human He failed to join the Super Smash Bros. Ultimate roster and used the Power of Rejection, but he has since been neutralized and turned physical. Currently, he is sometimes seen running a taco stand.

Male Species: Super Meme Guardian Founder of Snitch Productions, which went bankrupt. He is wicked and has tsundere tendencies. He possesses the YouTube Remote, a universal remote control. He was formerly the head of Snitch Productions, but now... he is the leader of the Internet Graveyard.

Male Species: Unknown Otaku He loves waifus very much.

Male Species: Garo He was previously imprisoned alone in a dungeon by Ganondorf for 500 years. This led him to crave attention from others, but since his attempts to get attention weren't well-received, he developed a desire to become a world-famous star. He is already an ally, but his rapper tendencies remain, which is why he came to the Villain School.

Male Species: Unknown College Student Driven by a desire to live in a more perfect world, he used his best friend SMG0 as a host to gain a powerful body through the power of the God Box. He destroyed worlds one by one by killing the Avatars that serve as the core of each universe to create his own new world. However, the worlds have been restored.

Male Species: Donkey Kong Resembles Funky Kong. A lawyer who separated the world with a barrier because of SMG4's memes; he appeared to enforce SMG4's copyright laws.

Male Species: Cardboard? Since he is made of cardboard, he communicates via radio. He moves around in an electric wheelchair. Marty stole SMG3's notebook after hearing false information from Mr. Puzzles that it contained a pizza recipe.