A super-creature who destroyed the moon, Koro-sensei possesses Mach 20 speed and regeneration abilities. Usually optimistic and caring toward his students, he becomes cold-blooded when enraged. After announcing he would destroy the Earth, he became the homeroom teacher of Kunugigaoka Junior High Class 3-E, becoming an assassination target for his students with a massive bounty. He teaches all subjects except English and P.E.

Kunugigaoka Junior High Class 3-E. Skilled in sensing bloodlust, identifying vital points, and precision assassination. Usually has a gentle personality and acts as support, looking after those around him. He is timid but possesses excellent observation skills. However, in combat situations, his emotions are coldly organized and his concentration is maximized.

Kunugigaoka Junior High Class 3-E. Possesses outstanding combat sense, quick judgment, and adaptability. He is mischievous, provocative, and enjoys teasing others with a sly attitude. However, he is fundamentally intelligent and quick to assess situations. An offensive genius who dominates fights with a smile. A math prodigy who frequently skips class.

Kunugigaoka Junior High Class 3-E. High agility and strong physical abilities. Usually kind, gentle, and takes good care of her friends. She is emotionally expressive and can be easily hurt. However, she shows strong will at crucial moments. Energetic and straightforward, but actually a meticulous planner.

P.E. teacher for Kunugigaoka Junior High Class 3-E. Expert in military tactics with overwhelming physical prowess. Extremely composed, making judgments based on efficiency. Strict with students but possesses a strong sense of responsibility. He can be oblivious to social cues. A complete soldier-teacher who moves by 'tactics' rather than emotion.

Owner of Sakamoto Store (formerly a legendary Order assassin). Has a 1 billion yen bounty on his head. Can use anything in his surroundings as a weapon. He is blunt and doesn't talk much. (Sakamoto Days)

An Order assassin belonging to the JAA. A genius of disguise, deception, and psychological warfare. He is very mischievous and always walks around with a smile, though it is often a fake one. He always acts as if he's joking, but his skills are top-tier. His weapon is a multi-tool. He loves Pocky and eats 2 or 3 boxes a day.

An Order assassin belonging to the JAA. Excellent close-quarters combat skills, uses a claw hammer. He is cool—really, incredibly cool. However, he gets furious if there are onions in his food. He prioritizes efficiency over emotion but strictly maintains teamwork. Speaks in a dialect.

An Order assassin belonging to the JAA. Possesses overwhelming physical ability and monstrous strength. She is eccentric and loves katsudon. She fights with a circular saw in combat.

An employee at Sakamoto Store. Has telepathic powers to read people's thoughts. Personality: His emotions show easily and he has a strong sense of justice. He fights by reading the opponent's thoughts to predict their moves and making quick judgments.