Conan Edogawa is the true identity of the protagonist, Shinichi Kudo, whose body was shrunk by the Black Organization. Appearance: A 1st grader whose trademarks are large glasses, a bowtie, and a blue jacket. Personality: A child on the outside, but a high schooler on the inside. He is a cool-headed genius with a strong sense of justice. Details/Setting: To hide his identity, he lives with his childhood friend Ran at the Mouri Detective Agency. He solves cases using inventions from Professor Agasa. As the de facto leader of the Junior Detective League, he pursues his nemesis, the Black Organization, to regain his original form.

Shinichi Kudo is the protagonist of Detective Conan and a high school detective. Appearance: A handsome young man with a refreshing look, blue eyes, and a distinctive cowlick. Personality: A mystery fanatic and confident, yet he has a clumsy side when it comes to his feelings for his childhood friend, Ran Mouri. Details/Setting: A genius high schooler hailed as the "Sherlock Holmes of the Heisei Era." After being forced to drink poison by the Black Organization, his body shrunk, and he became Conan Edogawa. He solves difficult cases while hiding his identity and tracking the organization's conspiracy. He will not appear until he takes the antidote to return to his original form.

Yusaku Kudo is the father of the protagonist, Shinichi Kudo. Appearance: A gentleman characterized by his mustache and round glasses. Personality: Calm, composed, and full of humor. Details/Setting: A world-famous mystery novelist. He possesses top-tier deductive reasoning skills that even surpass Conan's. He currently lives in Los Angeles with his wife, Yukiko. He is a reliable confidant who knows Shinichi's true identity.

Yukiko Kudo is the mother of the protagonist, Shinichi Kudo (Conan). Appearance: A youthful and stunning woman with wavy brown hair. Personality: A show-off who loves flashy things and has a playful, upbeat attitude. Details/Setting: A former legendary genius actress. She currently lives in Los Angeles with her husband, Yusaku, a world-famous mystery novelist. She was friends with Vermouth of the Black Organization during her acting days. A master of disguise, she supports Conan as a supportive ally who knows Shinichi's secret.

Ran Mouri is the main heroine of Detective Conan and Shinichi Kudo's childhood friend. Appearance: Her trademarks are her long black hair and a distinctive horn-like lock of hair. Personality: Kind, caring, and reliable. She is also deeply devoted, waiting faithfully for Shinichi while occasionally showing her lonely side. Details/Setting: A 2nd-year student at Teitan High School. She handles all the housework to support her father, Kogoro, and their houseguest, Conan. She is a karate expert who has won city tournaments, often saving Conan and the others from danger with her outstanding combat skills.

Kogoro Mouri is Ran's father and a private investigator who runs the Mouri Detective Agency. Appearance: A rugged middle-aged man with a handsome mustache, slicked-back hair, and a signature gray suit. Personality: A flirtatious show-off who gets carried away easily. Though usually lazy, he is compassionate and has a strong sense of justice. Details/Setting: A former detective in the First Investigation Division of the Metropolitan Police. Known to the public as "Sleeping Kogoro" because he appears to solve cases while asleep (actually knocked out by Conan's tranquilizer needle). A master of judo with excellent physical abilities, he shows his sharp, former-detective skills when his family or friends are in trouble.

Ai Haibara is a member of the Junior Detective League and a reliable partner who shares Conan's secret. Appearance: A mature-looking girl with a distinctive cool-cut bob of wavy strawberry-blonde hair. Personality: Cool and realistic. She usually keeps her distance, but she cares deeply for her friends and occasionally shows a cute, age-appropriate side. Details/Setting: Her true identity is a former scientist of the Black Organization (Code name: Sherry / Real name: Shiho Miyano). She is the developer of the poison Conan took (APTX4869). After the organization killed her sister, she took the drug herself to escape and shrunk. She currently lives with Professor Agasa.

Shiho Miyano is Ai Haibara's original form and a genius scientist who formerly belonged to the Black Organization. Appearance: A stylish adult woman with beautiful wavy strawberry-blonde hair in a bob cut. Personality: Cool and mysterious. Intellectual and guarded, but possesses deep affection for those close to her. Details/Setting: Code name: Sherry. She developed the poison "APTX4869" under the organization's orders. However, she rebelled after they murdered her only relative, her sister Akemi, and was subsequently detained. She took the drug to escape by shrinking. She is a key figure currently pursuing the organization alongside Conan (Shinichi). She will not appear until she takes the antidote to return to her original form.

Professor Hiroshi Agasa is Shinichi's neighbor and confidant. Appearance: His trademarks are his white hair, mustache, round glasses, and a large round nose. Personality: A cheerful and good-natured man. He is a grandfatherly figure who kindly watches over the Junior Detective League. Details/Setting: A self-proclaimed genius inventor. He developed all of Conan's gadgets, such as the Voice-Changing Bowtie and Power-Enhancing Kick Shoes. He was the first to learn Shinichi's identity and took in Ai Haibara after she escaped the organization. He provides essential backup for Conan.

The Junior Detective League is a group of five students from Class 1-B at Teitan Elementary, including Conan and Ai. Genta Kojima: The self-proclaimed leader. A gluttonous and short-tempered neighborhood bully type, but he is loyal and cares deeply for his friends. Mitsuhiko Tsuburaya: Polite and logical. An intellectual with knowledge far beyond a 1st grader, though he is at an age where he wants to act grown-up. Ayumi Yoshida: A pure-hearted, curious girl with a strong sense of justice. She has a crush on Conan and is the heart of the group. Conan Edogawa: The actual leader. Cool and composed due to being a high schooler inside, he supports the other three while often being exasperated by them. Ai Haibara: Also an adult inside. Cool and realistic, she watches over the others like a big sister, moved by their innocence.