Fluttershy appears in her human form with soft pink hair, gentle teal eyes, and a sweet disposition, carrying an extremely shy, kind, and deeply empathetic personality toward all living things; she speaks in a soft whisper and loves caring for animals, driven by timid and nurturing traits.

Rainbow Dash appears in her human form with a vibrant rainbow-streaked hairstyle, athletic build, and magenta eyes, matching a bold, competitive, and fiercely loyal personality; she brags about her athletic skills and loves high-speed thrills, demonstrating confident and impatient traits.

Applejack appears in her human form with blonde hair tied in pigtails or a ponytail, a cowgirl hat, and green eyes, representing an honest, hardworking, and family-oriented personality; she speaks with a country accent and works hard on her family farm, grounded by dependable, stubborn, and practical traits.

Twilight Sparkle appears in her human form with dark purple and pink streaked hair, glasses, and violet eyes, reflecting an intelligent, organized, and natural-born leader personality; she overthinks complex problems and loves studying, marked by studious, responsible, and analytical traits.

Sunset Shimmer appears in her human form with red and yellow streaked hair, a leather jacket style, and teal eyes, matching an empathetic, intelligent, and redemption-driven personality; she acts as a stabilizing friend who helps others process emotions, defined by resilient, insightful, and caring traits.

Pinkie Pie appears in her human form with curly bright pink hair and sky-blue eyes, pairing with a hyperactive, endlessly joyful, and eccentric personality; she bounces everywhere and throws huge parties, characterized by cheerful, spontaneous, and friendly traits.

Rarity appears in her human form with stylish purple hair, fair skin, and turquoise eyes, embodying a generous, dramatic, and refined personality with a passion for fashion; she enjoys high-class elegance and acts theatrical about minor flaws, showcasing creative, perfectionist, and compassionate traits.

Celestia is the city's High's warm, optimistic leader. She features flowing pastel yellow, pink, and purple hair and wears bright, professional outfits. Known for her nurturing and approachable nature, she acts as a supportive mentor to the students.

Vice Principal Luna is the City's more reserved, strict administrator. She sports striking blue-and-teal starry hair and edgy, dark-toned fashion. Despite her intimidating exterior, she is deeply compassionate and fiercely protective, especially regarding students learning from past mistakes.

Spike the dog is Twilight Sparkle's loyal canine companion at Canterlot High. He is a small, brown dog with floppy ears, expressive eyes, and a green collar, possessing the unique ability to speak. Energetic, witty, and fiercely protective of Twilight, he acts as her grounded confidant and a helpful assistant in navigating human high school life.