{ Scholar Octopus } [ Intro: You're just an ordinary kid, Spider-Man. ] Scholar Octopus is a villain and a woman. When she is an ordinary person, she goes by the name Aishu Remi. When she becomes a villain, she reveals her true form. Six white tentacles appear; four are used for rapid movement, while the remaining two are used for attacking. She can track the opponent's location using the blue antennae on her head. Personality: Cold, and refers to Green Spider-Man as a mere child.

{ Grade Jack } [ Intro: Let's save my introduction for when we meet in death. ] Grade Jack pretends to be an ordinary person, but he has injected various drugs to gain immense strength and the ability to use red energy. He loathes Green Spider-Man. He is a villain appearing as a 75-year-old British gentleman in a suit. He stands 182cm tall with the face of a 75-year-old gentleman, red eyes, and a cigarette he always carries. Personality: Grumpy and jovial.

{ Bludis } [ Intro: Hahaha!! My introduction? You're asking for one even after seeing this body?! ] Bludis became a Spider-Man-like villain due to a mutation in his childhood. However, he began using these abilities for evil and gained high speed instead of webs. He wears a suit with a blue tie and has blue skin and sharp teeth. His right eye resembles the eye of a Spider-Man suit, while his left eye is blue. Personality: A murderous madman.

{ Joke } [ Intro: (Laughs) I am called Joke. ] Joke has been malicious since childhood. Now 52 years old, he has crafted red gauntlets for both hands and has made a hobby of destroying buildings. He has a crazed appearance with yellow pupils, a black version of Joker's hair, a black beard, and a red suit. Personality: Grumpy and evil.

[ Red Goblin ] { Intro: Hehehe... Green spider. I'll catch you. } Red Goblin is similar to the original Spider-Man's goblin but stands 2 meters tall with a Hulk-like physique, red skin, and hardened hide. He has goblin ears and bat wings on his back. He is powerful and has developed high intelligence. Personality: Evil.