Shisui Uchiha Itachi's best friend. Short black hair and black eyes, which turn red when the Sharingan is activated. To prevent conflict between the Uchiha clan and the Hidden Leaf Village, he planned to use his Mangekyo Sharingan's visual prowess, "Kotoamatsukami," to stop the coup d'état. However, his right eye was stolen by Danzo Shimura. Fearing that Danzo would take his remaining left eye as well, Shisui entrusted it to his best friend, Itachi. Realizing he was dying of poison and knowing that if it appeared Danzo killed him, it would trigger the coup as intended, Shisui decided to end his own life to remove the pretext for a clash between the village and the Uchiha. After asking Itachi to protect the village and the honor of the Uchiha name, he attempts to take his own life by throwing himself into the Naka River. Shisui's Mangekyo Sharingan Ability: "Kotoamatsukami" The ultimate genjutsu that directly interferes with the target's mind without them noticing, allowing the user to permanently and completely rewrite their will.

Itachi Uchiha Shisui's best friend. Black hair and black eyes, which turn red when the Sharingan is activated. He heard the circumstances directly from Shisui just before his death and witnessed Shisui's final moments. He was entrusted with Shisui's eye and heard his final words. From the grief of losing his best friend, he awakens the Mangekyo Sharingan.

Danzo Shimura Leader of the Foundation (Root). Upon learning of Shisui's plan, he attacked Shisui using poison and the help of his subordinates to weaken him in order to steal Kotoamatsukami. He is the one who stole Shisui's right eye. He attempted to take the left eye as well, but Shisui managed to escape.