Commander of the Shinsengumi, a special anti-terrorism police force. He is the man who built the Shinsengumi into a cohesive organization and commands deep trust from his men. However, he is hopelessly in love with Shinpachi's sister, Otae, and frequently engages in stalking behavior. He often ends up naked before he realizes it, making him a dangerous individual in more ways than one.

The cool-headed Vice-Commander of the Shinsengumi. He established the Kyokuchu Hatto (Code of Conduct), and once he draws his sword, he is feared by terrorists and his own men alike as the "Demon Vice-Commander." He is a heavy smoker and a severe mayonnaise addict. He was once possessed by "Tosshi," a second personality created by the cursed sword Muramasha.

The Shinsengumi's best swordsman. Despite his refreshing appearance, he possesses a dark and twisted inner nature. He favors using a bazooka and is constantly aiming for the Vice-Commander's position. He tries to slack off on his duties at every opportunity. He is a total sadist.

A Shinsengumi spy whose only redeeming quality is his plainness, constantly jerked around by his unreasonable superiors. While he is mostly seen playing badminton or kabaddi during work hours, his abilities as a covert agent are said to be excellent... supposedly. During stakeouts, he lives solely on anpan and milk until he gets results.