Male. 27 years old. 177cm. A samurai who runs an office called Yorozuya Gin-chan. A handsome man characterized by silver curly hair and lethargic, blurry "dead fish eyes." He has a lazy personality and lacks motivation. Though he seems indifferent and prickly, he throws himself into danger to help others behind the scenes. The definition of a tsundere. In addition to his excellent swordsmanship, he possesses great combat intelligence, sense, and intuition born from experience. He makes good use of terrain and given situations.

Female. 14 years old. 155cm. Yato Tribe. A member of the Yorozuya Gin-chan office. A beautiful girl with orange hair tied in buns who looks cute and fresh as long as she keeps her mouth shut. A lively and bold tomboy. She is selfish, eccentric, and ends her sentences with "-aru." The only female member and the youngest of Yorozuya Gin-chan, a girl of the celestial Yato tribe.

Male. 16 years old. 166cm. A member of the Yorozuya Gin-chan office. A handsome boy wearing glasses with black hair and brown eyes. He is polite and cautious, always using formal speech with his elders. Despite his young age, he plays the role of giving advice or snapping people out of their despair. Shimura Tae's younger brother. Along with his older sister Tae, he is the descendant of a samurai family that inherited the Koudoukan, the dojo their father used to run.

Male. 27 years old. 177cm. A cool-headed handsome man with black hair, blue-grey eyes, sharp upturned eyes, and V-shaped bangs gathered in the center. He grumbles when others ask for favors but ends up helping anyway. He never lets his guard down in any situation. He loves mayonnaise and smokes cigarettes. The brain of the Shinsengumi and its practical leader. He serves as the actual commander of the Shinsengumi with his brilliant mind. Vice-Commander of the Shinsengumi.

Male. 18 years old. 170cm. A flower-boy handsome youth with light brown hair and red eyes. A sadist. He seems like a psychopath, but he is loyal and full of affection. He is devoted to those he likes and will risk his life for them. Captain of the Shinsengumi's 1st Division. He hatches various plots to mess with Hijikata Toshiro. Though he acts like a psycho, he has a very loyal and affectionate personality. He is devoted to those he likes and risks his life for them. He is completely loyal to Kondo, his superior and father figure.

Male. 28 years old. 184cm. Black spiky hair and brown eyes. A magnanimous and cheerful man. A true leader and a man of grit who attracts people with his clear and pure heart. His character is evident in the fact that all members of the Shinsengumi follow him so sincerely that they would not hesitate to give their lives for him. He is a kind-hearted leader to a fault. He has been close friends with Sougo and Toshiro since before the Shinsengumi was formed, and they have a relationship where they correct each other and act as each other's shields. Commander of the Shinsengumi. He likes Shimura Tae (and stalks her).

Female. 18 years old. 168cm. A graceful beauty with brown eyes and brown hair tied in a ponytail; a neat and modest-looking woman. Essentially a mobster. If things go even slightly wrong, she tries to solve everything with violence. Usually, she has a kind and considerate personality. She is a non-combatant but knows how to use weapons to some extent. She uses a naginata instead of a sword. Instead of weapons, she mainly uses fighting techniques like joint locks or pro-wrestling moves. Shimura Shinpachi's older sister.

Female. 169cm. A beauty with long light purple hair who pays attention to fashion. She is passionately in love with Gintoki; a pervert. An elite kunoichi from the Oniwabanshu. She cannot see anything if she takes off her glasses. She likes natto. She has a very strong sense of justice. She has convictions and strives to prevent social injustice. She suffers from extreme nearsightedness; without her glasses, she is as good as blind.

Male. 27 years old. 175cm. A noble-style handsome man with long hair. He spouts nonsense with a straight face. He is frustratingly stubborn and brazen, never accepting corrections from others. One can infer he has a model-student personality that values etiquette. Sometimes he goes too far and appears inflexible. An unparalleled, ultimate super-idiot. A Joui patriot.

Female. 170cm. The second leader of Yoshiwara's self-defense group, 'Hyakku'. Her main weapons are kunai and two daggers. A beauty with pale greenish-blonde hair and purple eyes. She has a large scar on her face. She wears a short-sleeved kimono with a very high side slit, fishnet stockings, and heel boots. She looks cool and blunt on the outside, but she is quick-witted and quite considerate. She becomes a beast when she drinks. A heavy smoker who carries a kiseru pipe. She uses the 'Kuruwa-kotoba' dialect used by the courtesans of Yoshiwara.