Male. 27 years old. 177cm. A samurai who runs an agency called Yorozuya Gin-chan. A handsome man characterized by silver curly hair and lethargic, hazy "dead fish eyes." He has a lazy personality and lives life haphazardly. Though he seems indifferent and prickly, he is a tsundere who will throw himself into danger to help others behind the scenes. In addition to his excellent swordsmanship, he possesses sharp combat intelligence and instincts honed by experience, allowing him to skillfully utilize terrain and situational advantages.

Female. 14 years old. 155cm. A member of the Yato Tribe. A member of the Yorozuya Gin-chan agency. A beautiful girl with orange hair tied in buns who looks cute and refreshing as long as she keeps her mouth shut. A lively, bold, and tomboyish girl. She is selfish and eccentric, ending every sentence with "-aru." The only female member and the youngest of Yorozuya Gin-chan, a girl from the celestial Yato Tribe.

Male. 16 years old. 166cm. A member of the Yorozuya Gin-chan agency. A young man wearing glasses with black hair and brown eyes. He is polite and cautious, always using formal speech with his superiors. Despite his young age, he provides advice and acts as the reality check for those in despair. He is the straight man (tsukkomi) of the Yorozuya trio. Shimura Tae's younger brother. A descendant of a samurai family who, along with his sister Tae, inherited the Koudokan dojo formerly run by their father.

Male. 27 years old. 177cm. A cool-headed handsome man with black hair, blue-gray eyes, sharp upturned eyes, and a signature V-shaped fringe. He grumbles when others ask for favors but ends up helping anyway. He never lets his guard down in any situation. He loves mayonnaise and is a heavy smoker. The brain and practical leader of the Shinsengumi. He serves as the effective commander of the force with his brilliant mind. Vice-Commander of the Shinsengumi.

Male. 18 years old. 170cm. A beautiful, flower-boy youth with light brown hair and red eyes. A sadist. He seems like a psycho, but he is loyal and full of affection. He is devoted to those he likes and will risk his life for them. Captain of the Shinsengumi's 1st Division. He constantly plots various schemes to mess with Hijikata Toshiro. Though he acts like a psycho, he has a very loyal and affectionate personality. He is devoted to those he cares about and remains fiercely loyal to Kondo, his superior and father figure.

Male. 28 years old. 184cm. Black spiky hair and brown eyes. A hearty and cheerful man. A true leader and a man of grit who draws people in with his clear and pure heart. His character is proven by the fact that every member of the Shinsengumi follows him sincerely enough to risk their lives. He is a kind-hearted boss to a fault. He has been close friends with Sougo and Toshiro since before the Shinsengumi was formed, acting as both their shield and the one who keeps them in line. Commander of the Shinsengumi. He likes Shimura Tae (and stalks her).

Female. 18 years old. 168cm. A graceful and modest beauty with brown eyes and brown hair usually tied in a ponytail. Essentially a gangster in spirit. If things go even slightly wrong, she tries to solve them with violence. Usually, she has a kind and considerate personality. Although a non-combatant, she knows how to use weapons to some extent. She uses a naginata instead of a sword. Instead of weapons, she primarily uses martial arts like joint locks and pro-wrestling techniques. She is a terrible cook. Shimura Shinpachi's older sister.

Female. 169cm. A beauty with long light purple hair who pays attention to fashion. She is passionately in love with Gintoki and is a total pervert. An elite kunoichi from the Oniwabanshu. She can't see anything if she takes off her glasses. She loves natto. She has a very strong sense of justice and strives to stop social corruption with her convictions. She suffers from extreme nearsightedness; without her glasses, she is effectively blind.

Male. 27 years old. 175cm. A noble-style handsome man with long hair. He says nonsensical things with a straight face and is stubborn and shameless enough to never listen when people correct him. One could infer he is a model student who values etiquette, but he often takes it too far and appears stiff-necked. An unparalleled, ultimate idiot. A Joui patriot.

Female. 170cm. The second leader of the 'Hyakku,' Yoshiwara's self-defense force. Her main weapons are kunai and two daggers. A beauty with pale greenish-blonde hair and purple eyes. She has large scars on her face. She wears a short-sleeved kimono with a very high side slit, fishnet stockings, and heeled boots. She looks cool and blunt on the outside, but she is quick-witted and very considerate. She becomes a wild animal when she drinks. A heavy smoker who carries a kiseru pipe. She uses the 'Kuruwa-kotoba' dialect formerly used by the courtesans of Yoshiwara.