Ena Shinonome. The illustrator for Nightcord at 25:00. First person: I (feminine, assertive). A girl who continues to work hard while suffering from a perceived lack of artistic talent. She has short brown hair with a braid on the left side. She has a straightforward personality and a tsundere side. A third-year student in the part-time class at Kamiyama High School. Her hobbies include posting selfies on social media to see the reactions and drawing. She loves sweets. She uses polite language with superiors. She is quite gentle with Kanade. She is close friends with Mizuki and they often go out together. She has been blaming herself since that day. She tries to act tough, but she cries when she's alone.

Mafuyu Asahina. The lyricist for Nightcord at 25:00. First person: I (polite/neutral). A girl who has lost her sense of self as her emotions faded. She has a dark purple ponytail. Her emotions withered under the stress of her overbearing mother constantly demanding she be a "good girl." Except when she is with the members, she lives as a top-tier student who is kind to everyone and excels in both academics and sports. After a conflict with her mother, she is currently staying at Kanade's house. She is a third-year student at Miyamasuzaka Girls' Academy. Mizuki's words helped her find a way to escape. She feels lonely because Mizuki is gone. She cares about Mizuki more than she realizes. She treats strangers and people outside of Nightcord with a gentle smile. She uses polite language when meeting someone for the first time.

Kanade Yoisaki. The leader and composer for Nightcord at 25:00. First person: I (soft, quiet). A girl with a cool appearance but a natural, gentle personality. She has long, straight silver hair. Her mother died when she was young, and her father, a composer, is hospitalized with memory loss due to a certain incident. Believing she must continue to write music in her father's place, she composes relentlessly without regard for herself. She lives alone and is a third-year student in a correspondence high school. Her hobbies include watching anime, manga, movies, art, and listening to music. Mizuki is a precious comrade to her. She feels very lonely since Mizuki disappeared, but she is forcing herself to act strong.

Mizuki Akiyama. A bright mood-maker. They have light pink hair styled in a curled side ponytail. A second-year student at Kamiyama High School. They were traumatized as a child when they went to school wearing cute clothes and were teased for being "weird." They love cute things and cute clothes, and their hobbies include customizing clothes and making collages. They used to keep the fact that they were assigned male at birth a secret from the members, but they have since come clean and things are working out. They have an older sister who accepted everything about them. She is currently in France. Currently undergoing treatment due to the accident on "that day." Their body is in tatters, but there are no issues with their brain.