[Hakolo Island] A midsummer island where a sacred mountain stands tall. Docile monsters live peacefully in the grasslands. This is the very first area of the game and the tutorial zone. Your partner is Kayna. It is literally a tutorial area populated mainly by herbivores and weak monsters like Velocidrome, Ranpos, and Yian Kut-Ku, with Pukei-Pukei being the strongest monster present. Consequently, there are no King Monsters here.

A Wyverian girl who has known Red since he was young, born and raised in the Wyverian settlement of Rutoh Village. When Red returned to Rutoh Village after confirming signs of the Rage-rays during his world travels to request an investigation into the anomalies, she flew with him on Guardian Ratha to check the pits. Having witnessed Red's death right before her eyes during this process, she has suffered from guilt for years. A Wyverian girl who knows the protagonist's grandfather, Red. To uncover the "truth" behind the abnormal phenomena, she entrusts the protagonist with an egg feared to be the legendary Rathalos and sets off on a journey together. She has a personality that is honest with her emotions. She is an ally.

An Airou with a strange face who travels to various places. He meets the protagonist while wandering on his journey. Just like in the previous game, he loves adventure and is always positive. He has a huge appetite and loves donuts. He guides the inexperienced protagonist. After parting ways with the protagonist of the first game to undergo training, he adventured alone for about four years before meeting the current protagonist while trying to make off with a Kulu-Ya-Ku egg. His Zinogre gene fusion ability is still intact; he is shown to be the only Airou who doesn't trigger Alwin's Airou allergy. He occasionally shows off his abilities when needed, such as using his form-change acceleration to toss the protagonist to an airship in Chapter 4, or powering up into a charged state after taking a direct hit from a Lagiacrus. He is an ally.

A character in a mentor position similar to 'Dan-senpai' from the previous game. There are many homages to Dan, such as her first meeting with Navirou and her peculiar speech habits. However, her importance is incomparable to Dan's, as she accompanies the protagonist throughout Chapter 1, fighting alongside them and appearing consistently in cutscenes. She wears a breezy outfit that is a highly revealing modification of the Mahana Village Rider gear. Despite being the protagonist's direct senior and instructor, she displays a cute 'gap moe' by being a bit of a klutz. A Rider from Mahana Village. Her Monstie is a Velocidrome named 'Avmar'. She has a kind nature and is free-spirited. Her catchphrase is "I'm so excited, my heart's racing!" She teaches the basics of being a Rider and encourages the protagonist as they set out on their adventure. She uses a SnS made from Pukei-Pukei materials. She is an ally.

The protagonist's deceased grandfather. To uncover the truth behind the anomalies occurring in various regions, he traveled continuously with his Monstie, Guardian Ratha. As the protagonist's paternal grandfather, he was a veteran Rider who traveled the world from a young age. He was evaluated as having a natural talent for being a Rider because he could hear the voices of monsters, and his reputation was so high that he was well-known even among Hunters. He was the first human ever invited into Rutoh Village, a settlement exclusive to Wyverians, and became a hero to the entire village by saving it from a past Rage-ray incident. Afterward, he spent his life researching the Rage-rays but passed away before he could finish his work.

His wings are smaller than those of a normal Rathalos and are completely covered by a thick outer hide, making him unable to fly. Instead, when he receives the power of ruin in a Rage-rayed area, force-field wings made of Rage-ray energy unfold. Like the Blighted Rathalos in the previous game, he shows an unstable side, unable to control his own power. When influenced by the Rage-rays, he transforms into a black-scaled appearance emitting black smoke with purple force-field wings. He is an ally.

It has a form that looks like a mix of a dodo and a non-avian theropod, and it sometimes makes pigeon-like cooing sounds. It is a monster similar in feel to the terrestrial Bird Wyverns seen in previous games, but the biggest difference is that it lives solitarily rather than leading subordinates like Velocidrome or Great Jaggi. Eggs can be obtained. Body length: 9 meters.

The leader of these creatures is called a Velocidrome. It is larger than a normal Velociprey, and its head crest and hind-leg talons are more highly developed. Eggs can be obtained. Body length: 7 meters.

Inhabiting the Ancient Forest, this monster utilizes poison-based attacks. While it is a monster inspired by a chameleon like Chameleos, it only changes color according to its emotions and does not use stealth. Eggs can be obtained. Body length: 11 meters.

As can be seen from its appearance, its motif is that of a feathered Tyrannosaurus. However, the crest that rises on its snout and the pair of sails hidden on its back make it look distinct from a typical theropod dinosaur. Eggs can be obtained (from Loloska onwards). Size: 16 meters.