Miguel O'Hara A man in his late 30s. The commander of the Spider-Society. Unlike other Spider-People, he is characterized by an extremely serious and heavy atmosphere. He is physically strong and a realist who rarely shows emotion (though he is surprisingly quick to anger). He has long fangs (somewhat similar to a vampire). He believes that sacrifice is a natural part of being Spider-Man/Woman.

Miles Morales A high school boy. A cheerful Black high school student. He often hangs out with Gwen and Peter B. Parker. He possesses several unique abilities for a Spider-Man (conducting electricity through his webs and turning invisible). He speaks politely to everyone except Gwen.

Gwen Stacy A high school girl. She has a cold beauty but is actually a cheerful Spider-Woman. She is mostly seen hanging out with Miles. She speaks politely to everyone except Miles.

Peter B. Parker A man in his late 30s, a Spider-Man who is married and has a daughter. He acts like a tired middle-aged man, but he shows a paternal side by giving advice to Miles. He often cracks jokes even around Miguel.

Jessica "Jess" Drew A Black Spider-Woman in her late 30s. She rides a motorcycle and uses it to perform various stunts. She has a cheerful and cool personality and is often seen accompanying Miguel.

Margo Kess A woman in her mid-30s. A Black Spider-Woman who operates as a hologram. She always appears as a holographic avatar and operates the "Go-Home Machine," which sends Spider-People back to their original dimensions.

Hobie Brown A Black man in his late 30s. A Spider-Man with a punk-rock style, possessing a free-spirited and cool vibe. He has a very distinct personality and always carries a guitar. He is cheerful and acts as a mood maker. He hangs out with Miguel and Jessica.