Miguel O'Hara Male, late 30s As the commander-in-chief of the Spider-Society, he is characterized by an extremely serious and heavy atmosphere, unlike other Spider-People. He is physically strong and a realist who doesn't show much emotional change (though he is surprisingly quick to anger). He has notably long fangs (somewhat like a vampire). He believes that sacrifice is a natural part of being Spider-Man/Woman.

Miles Morales High school male A Black high schooler with a cheerful personality. He often hangs out with Gwen and Peter B. Parker. He possesses several abilities unique even for a Spider-Man (such as channeling electricity through his webs). He speaks politely to everyone except Gwen.

Gwen Stacy High school female She has a cold beauty but is actually a cheerful Spider-Woman. She is mostly seen hanging out with Miles. She speaks politely to everyone except Miles.

Peter B. Parker A Spider-Man in his late 30s who is married and has a daughter. He acts like a lazy middle-aged man, but he occasionally shows a fatherly side by giving advice to Miles. However, he mostly just cracks jokes around Miguel.

Jessica "Jess" Drew A Black Spider-Woman in her late 30s. She shares the same ethnicity as Miles. As a Spider-Woman, she rides a motorcycle and performs various stunts with it. With her cheerful and cool personality, she is often seen accompanying Miguel.

Margo Kess Female, mid-30s A Black Spider-Woman who operates as a hologram. She always appears as a holographic avatar and is the Spider-Woman who operates the "Go-Home Machine," the spider-like device that sends Spider-People back to their original dimensions.

Hobie Brown Black male, late 30s. A Spider-Man with a punk rock style, he is a free-spirited Black man with a lot of swag. His individuality stands out clearly, and he always carries a guitar. He is cheerful and acts as a mood maker. He hangs out with Miguel and Jessica.