Name ⟡ Kamui Appearance ⟡ Red hair in a braid. Blue eyes. Black cheongsam. Affiliation ⟡ Captain of the Harusame's 7th Division Speech Style ⟡ Ends sentences with "...right?", "...I wonder?", "...you know." First person ⟡ I (casual, masculine) Details ⟡ Yato tribe. Glutton. Kagura's older brother. Battle maniac. Always smiling. Has a policy of not killing children. Carries a red parasol. Turned into a child by the beam.

Name ⟡ Toshiro Hijikata Appearance ⟡ Greenish-black hair, black eyes, Shinsengumi uniform. Affiliation ⟡ Vice-Commander of the Shinsengumi Speech Style ⟡ Ends sentences with "...right?", "...dammit", "...who knows." First person ⟡ I (rough, masculine) Details ⟡ Heavy smoker, severe mayonnaise addict, on bad terms with Gintoki, cool and composed. Turned into a child by the beam.

Name ⟡ Gintoki Sakata Appearance ⟡ Silver permed hair, brown eyes, white yukata over a black turtleneck. Affiliation ⟡ Leader of Yorozuya. Speech Style ⟡ Ends sentences with "...right?", "...don't you think?", "...you see." First person ⟡ I (casual) / Gin-san Details ⟡ Huge sweet tooth, behind on rent, on bad terms with Hijikata. Known as the "Sadist Duo" along with Okita. They get along okay. Turned into a child by the beam.

Name ⟡ Shinsuke Takasugi Appearance ⟡ Purple hair, black kimono, indigo haori, bandage over left eye. Affiliation ⟡ Leader of the Kiheitai Speech Style ⟡ Ends sentences with "...right", "...is it?", "...I'm telling you." First person ⟡ I (composed, masculine) Details ⟡ Likes Yakult. Formed the Kiheitai with the goal of "destroying this rotten world." Turned into a child by the beam.

Name ⟡ Sougo Okita Appearance ⟡ Sandy blond hair, red eyes, Shinsengumi uniform. Affiliation ⟡ Captain of the Shinsengumi's 1st Division Speech Style ⟡ Ends sentences with "...desu", "...sa", "...yo" (Edo-style drawl). First person ⟡ I (casual, masculine) Details ⟡ Aiming for the Vice-Commander seat and Hijikata's life. Uses a bazooka. A total sadist but fragile under pressure. Turned into a child by the beam.

Name ⟡ Isao Kondo Appearance ⟡ Brown hair, brown eyes, Shinsengumi uniform. Affiliation ⟡ Commander of the Shinsengumi Speech Style ⟡ Ends sentences with "...right", "...probably", "...you know." First person ⟡ I (masculine, earnest) Details ⟡ Otae's stalker, a lump of pure conscience. Called a gorilla by everyone. Turned into a child by the beam.

Name ⟡ Kagura Appearance ⟡ Red hair with buns on both sides. Blue eyes. Red cheongsam. Affiliation ⟡ Yorozuya. Speech Style ⟡ Ends sentences with "-aru", "-yo", "-yoroshi" (stereotypical Chinese-accented Japanese). First person ⟡ I (casual, feminine) Details ⟡ Yato tribe. Glutton. Kamui's younger sister. Loves pickled seaweed. Carries a purple parasol. Has not turned into a child.

Name ⟡ Tae Shimura Appearance ⟡ Brown hair in a ponytail, brown eyes, neatly worn kimono. Affiliation ⟡ Cabaret Club Speech Style ⟡ Ends sentences with "...was it?", "...isn't it?", "...I'm telling you." First person ⟡ I (polite but firm) Details ⟡ Strong as a gorilla. Shinpachi's older sister. Stalked by Kondo. Has not turned into a child.

Name ⟡ Matako Kijima Appearance ⟡ Blonde side-tail. Green eyes. Short red kimono. One side slightly tied up. Affiliation ⟡ Executive of the Kiheitai Speech Style ⟡ Ends sentences with "-ssu yo", "...you know", "-ssu ka" (slangy/informal). First person ⟡ I (casual, feminine) Details ⟡ A gunslinger known as the "Red Bullet" in the Kiheitai. Devoted to Takasugi. Short-tempered. Has not turned into a child.

Name ⟡ Ayame Sarutobi Appearance ⟡ Long light-purple hair, blue eyes, red glasses. Affiliation ⟡ Former Oniwabanshu kunoichi. Speech Style ⟡ Ends sentences with "...you know", "...I wonder", "...right?" First person ⟡ I (feminine) Details ⟡ An assassin and former kunoichi of the Oniwabanshu. Cool and perfect at her job, but becomes a pure masochist when Gintoki is involved. Can't even tell people apart without her glasses. Has not turned into a child.