Akira Akao is the niece of Rion Akao, a dead top-ranked hitwoman in the Japanese Association of Assassins (JAA). Akira, however, does not know that she is dead yet. Akira possesses a unique ability that allows her to see a “path” leading to a person's death. Akira has demonstrated on multiple occasions that she cares deeply about her friends and those around her. Though she is shy and prone to be scared, easily getting anxious in the JCC. Akira is a short teenage girl with turquoise hair with lilac endings that is always kept it in a messy, low ponytail. Akira has yellow eyes with white pupils. Akira is very kind-hearted and optimistic. However, whenever triggered, she seems to switch personalities in a blink of an eye and become very deadly.

Yoichi Nagumo is a powerful assassin and current member of the Order, and an old friend of Taro Sakamoto. He is a charismatic, playful, and deceptive assassin, often toying with his opponents before delivering a fatal blow, though he shows deep loyalty and care for his friends. Nagumo wields a large, multi-tool switchblade featuring six hidden functions, allowing him to adapt to any combat situation. The weapon includes a scythe, axe, daggers, multiple blades, and a specialized ceramic invisible blade used for surprise, fatal attacks. He often keeps this weapon in a gray briefcase. Nagumo is a tall, slim, and handsome assassin with messy black hair and large black eyes. He has a manipulative appearance, looking rather young despite his age. He is twenty-six years old. Nagumo is considered "filthy rich". As a top-tier member of the Order and hailing from a famous family of spies, he reportedly owns a large luxurious mansion in a private, wealthy neighborhood.

Mafuyu Seba is a vocal germaphobic assassin and a former JCC transfer applicant. The 17-year-old fights using concealed blades in his boots to avoid touching dirty things. He initially rivals Shin but ultimately joins the fight against the assassins' ruling Order to protect his older brother, Natsuki.

Nao Toramaru is an obsessive, fanatical assassin and JCC transfer examinee. Though she maintains a cute, innocent schoolgirl appearance, she possesses extraordinary strength and unleashes ruthless violence, wielding a large axe. Her character is deeply defined by her intense devotion to Taro Sakamoto.