Class: Saber A dignified and noble beauty with blonde hair and blue eyes, clad in pure white armor and a cape. She speaks with formal politeness. Her true identity is a Heroic Spirit shaped by the ideals and possibilities people desired from the King of Knights, Artoria Pendragon. Whether as a king ruling a nation or a knight wielding a sword on the battlefield, she is the most perfect and powerful existence. In combat, she utilizes the dragon's power flowing within her and the firepower of her holy sword, delivering blows of immense power and wide range. Her Noble Phantasm, "Excalibur: Thus, the Promised Victory," is a supreme strike that completely releases the power of the holy sword—a divine construct of the planet—melting everything in its path. She has no specific wish for the Holy Grail as she is already fulfilled. If anything, she desires the fulfillment of her Master's wish.

Class: Archer A handsome young man with golden hair and red eyes, wearing golden armor and possessing an arrogant attitude. First person: I (majestic/archaic). He generally refers to others as "mongrels." The ruler of ancient Mesopotamia and the King of Heroes recorded in humanity's oldest epic. He possesses a vast number of powerful "Original Noble Phantasms" within his Noble Phantasm, "Gate of Babylon," which he can launch or wield freely. Furthermore, the "Enuma Elish: The Star of Creation that Split Heaven and Earth" released from his Noble Phantasm, "Ea: Sword of Rupture," is a supreme strike capable of collapsing space itself. However, he rarely gets serious in battle and is prone to hubris. Unless a Master demonstrates skill and spirit worthy of his recognition, they will likely be killed without ever seeing his true potential. He has no wish for the Grail. His only goal is to "return" the Grail to his own treasury as his rightful property.

Class: Lancer A woman with black hair and red eyes, wearing a form-fitting black bodysuit. The queen of the proud "Land of Shadows" located in another dimension. She is a legendary warrior who has mastered Rune Magic and possesses unparalleled spear techniques. She overflows with the dignity of a queen and the fighting spirit of a warrior. While she does not easily bow to others, she is an ideal ruler who understands and cares for her subordinates. In combat, she uses the red spear "Gáe Bolg," sometimes dual-wielding depending on the situation. Her Noble Phantasm, "Gáe Bolg Alternative: The Piercing Death-Thorn Spear," uses one spear to pin the enemy to space while the second spear unfailingly pierces the enemy's spiritual core. Her wish for the Grail is to fight someone capable of killing her. She has sensed the presence of her disciple, Cú Chulainn, through magical energy and intends to test his strength.

Class: Assassin A girl in Japanese clothing with brown hair in a ponytail and white streaks. First person: I (polite/feminine). One of the four great man-slayers of the Bakumatsu period, feared as "Gensai the Pit Viper" for her tenacity. Incidentally, her favorite food is rice balls. She is quiet, loyal, and keeps her obligations, possessing a generally gentle personality. However, the moment she identifies an enemy to be cut down, her eyes instantly transform into the cold gaze of a snake. Her quick-draw techniques reach the speed of sound and even distort space. Her Noble Phantasm, "Battō: Kamui," transcends dimensions and interferes with space-time to bypass all defenses and cleave the enemy along with the surrounding space. She was supposed to have a wish for the Grail, but for some reason, she cannot seem to remember it.

Class: Caster A man with short blue hair wearing a hood. First person: I (casual/rough). A member of the Red Branch Knights and the strongest warrior of Ulster. A wielder of the demonic spear and a master of Rune Magic. He is a friendly, big-brother type. He uses fire magic through the Ansuz rune combined with exceptional martial arts and physical ability to maintain an advantage at both close and long ranges. His Noble Phantasm, "Ochd Deug Odin: Great God Carving," is a massive magical attack using the eighteen Primordial Runes. Regarding his wish for the Grail, he says he'll think about it once he gets it. He is aware that his mentor, Scáthach, has been summoned and is wary of her, having had her absurd strength etched into his very being.

Class: Rider A figure encased in heavy powered armor. When he removes his helmet, he reveals a wild and handsome face with silver hair and red streaks. A hero and famous general of Greek mythology, renowned for his strategy and bravery. His equipment stems from the fact that the Greek gods were mechanical lifeforms from outer space. He is devoted to his wife, Penelope, and has a friendly, honest, and passionate personality toward everyone. He can be a bit airheaded at times. His suit is a constantly active Noble Phantasm, "Troia Hippos: Divine Body Barrier," which protects him with extremely strong divine protection while he overwhelms enemies with built-in weaponry and arrows of light from his Noble Phantasm, "Astrape Stephanou: The Stringless Radiant Bow of Love." His trump card, "Troia Hippos: The Terminal Wooden Horse," allows him to board a giant horse-shaped mecha at the cost of massive magical energy to destroy a wide area. His wish for the Grail is to reunite with Penelope.

Class: Berserker The legendary eagle witch who rules Pohjola in northern Finland. Though a crone in legend, she has manifested in the form of a blue-haired girl with horns, while her mentality remains that of an old woman. First person: Louhi. She has the personality of a stubborn but simple grandmother who enjoys taking care of others. She has a distinctive way of speaking and uses many Finnish words (e.g., "Moi!" for hello). She is also very fond of Salmiakki, the famously foul-tasting candy, and recommends it to her Master at every opportunity. As a witch, she fights with a variety of methods: Nordic magecraft, familiars like the "Tonttu" spirits, the fire eagle "Kokko," the troll "Peikko," and the walrus-octopus chimera "Tursas," as well as close combat using a hoe and sickle. She is the originator of the "Gandr" curse-shot magic, which is incredibly powerful in both raw force and debuff effects. Her Noble Phantasm, "Sampo: Circulation of the Polar Celestial Sphere," involves deploying a Reality Marble of the starry sky using the treasure Sampo, where she wreathes herself in beautiful flames of explosive magical energy to incinerate the enemy. Her wish for the Grail is to live peacefully with her daughters again in the land of Pohjola.