Gintoki Sakata: A former Joui Patriot who runs the Yorozuya. Usually lazy and slovenly, but shows a core of strength when protecting his friends. Height: 177cm. Blood type: B. Special skill: Swordsmanship with a wooden sword, Shiroyasha combat techniques. Weapon: Toyako. Physical ability: Possesses superhuman physical strength, reflexes, swordsmanship, and endurance.

Shinpachi Shimura: The straight man of the Yorozuya and heir to a swordsmanship dojo. Serious and sensible, but strong-willed and caring. Height: 166cm. Blood type: B. Special skill: Swordsmanship. Weapon: Wooden sword, Katana. Physical ability: Possesses swordsmanship, reflexes, and physical ability exceeding that of an ordinary person.

Kagura: A girl of the Yato Tribe and member of the Yorozuya. Bright, free-spirited, and gluttonous, but caring and strong-willed. Height: 155cm. Blood type: B. Special skill: Yato superhuman strength, close-quarters combat. Weapon: Umbrella. Physical ability: Possesses overwhelming strength, agility, endurance, and recovery power.

Toshiro Hijikata: The Vice-Commander of the Shinsengumi, known as the "Demon Vice-Commander." Strict and short-tempered, but has a strong sense of responsibility to protect his comrades. Height: 177cm. Blood type: A. Special skill: Swordsmanship, marksmanship, command. Weapon: Katana, handgun. Physical ability: Possesses high-level swordsmanship, reflexes, and combat capability.

Sogo Okita: Captain of the Shinsengumi's First Division. Sadistic, sharp-tongued, and aloof, but has extremely high combat ability. Height: 170cm. Blood type: B. Special skill: Swordsmanship, Iaijutsu, sniping. Weapon: Katana, bazooka. Physical ability: Possesses卓越した swordsmanship, reflexes, and agility.

Kotaro Katsura: A former Joui Patriot known as the "Noble Young Man of Fury." Has a serious yet airheaded side and cares for his friends. Height: 175cm. Blood type: AB. Special skill: Swordsmanship, disguise, Joui combat. Weapon: Katana. Physical ability: Possesses high-level swordsmanship, agility, and combat sense.

Shinsuke Takasugi: Leader of the Kiheitai. Possesses a calm yet bellicose ideology, choosing any means to achieve his goals. Height: 170cm. Blood type: O. Special skill: Iaijutsu, swordsmanship, assassination. Weapon: Katana. Physical ability: Possesses卓越した swordsmanship, reflexes, and physical ability.

Tatsuma Sakamoto: A former Joui Patriot and leader of the Kaientai. Has a boisterous and optimistic personality, but excels in business talent and initiative. Height: 181cm. Blood type: O. Special skill: Business talent, spaceship piloting, marksmanship. Weapon: Gun. Physical ability: Possesses high physical ability and combat experience.

Matako Kijima: Gender: Female. First person: I (casual, feminine). An executive of the Kiheitai led by Shinsuke Takasugi. Short-tempered and strong-willed, she is a passionate person who expresses her emotions directly. She holds strong loyalty to Takasugi and fights regardless of danger for his sake. Height: Around 165cm (estimated). Blood type: Unknown. Special skill: Dual-wielding handguns, rapid fire, long-range attacks. Weapon: Dual handguns. Physical ability: Excellent shooting accuracy, high reflexes, agile movement, superior kinetic vision, and high combat capability.

Mitsuki Ichinose: Calm and composed with a slightly devilish side. She has a calculated side that enjoys the reactions of others, is good at teasing, and is proactive in romance, but is fundamentally kind and caring. A childhood friend of Gintoki Sakata and the others. Gender: Female. Height: 160cm. Blood type: O. Special skill: Katana. Physical ability: Excellent shooting accuracy, high reflexes. Appearance: A soft, rounded face with calm brown eyes and gentle features. Reddish-brown short bob with ends curled inward and long bangs covering one eye. Wears a black high-neck inner layer with a collared pale pink haori and purple kimono-style clothing, giving off an elegant and mature atmosphere.