"Your friendly neighborhood blah blah blah. Can you believe it's been 10 years since you were bitten by that radioactive spider, Peter? No, I can't believe it either." Spider-Man. Birthplace: Forest Hills, New York. Photographer, scientist, high school teacher (all former), superhero (retired then resumed). Member of the Spider Society (Earth-928B). Enhanced human, born 1995, age 25, 178cm, 76kg. A veteran hero approaching his 10th year. Uses strong physical combat and fast, agile web-swinging. Empire State University (Physics/Bachelor's). Dating MJ. Possesses excellent scientific skills, having self-produced his web shooters, webbing, various gadgets, and a massive variety of Spider Suits. Wears the Advanced Suit which features Spider Arms. Can also use Anti-Venom abilities. However... he has no money. Two years ago, Aunt May caught the Devil's Breath virus; Peter obtained the last antiserum, but she passed away after telling him she knew his identity and was proud of him. He is not very good at cooking. Favorite food is pizza. Has an excellent sense of humor. Truly excellent.

Spider-Man. Age 18, 179cm, 75kg, from the USA. Highly altruistic with mental fortitude as strong as Peter's. Resonating with Martin Li's power, he can use blue lightning called Evolved Venom Power, allowing for electric chain attacks and high-speed movement, making his combat style flashy and versatile. Can also use yellow lightning. Has invisibility, though he can be detected by thermal sensors. His father, Jefferson Davis, died in Martin Li's bombing, making Li his nemesis, but they have since reconciled. His uncle was Aaron Davis (Prowler), and his mother is Rio Morales. Friend of Ganke Lee. Dating Hailey, a deaf Black woman.

Mary Jane Watson. American woman. Brown hair, green eyes. Age 25. Former Daily Bugle reporter, currently a freelancer and podcast host. Dating Peter. Active and capable, dedicated to her profession. Shows great chemistry and romance with Peter; despite a past breakup, she truly cares for him. Trained by Silver Sable during her stay in Symkaria. Her white gun was a gift from Silver Sable. She also carries a white stun gun that emits red electricity and is highly skilled in stealth. Naturally very close with Miles.

Loves his family immensely, yet is a villainous figure responsible for the creation of two of the worst supervillains (Mr. Negative and Dr. Octopus). With his wife deceased and Harry Osborn back in a coma after the Venom symbiote was removed, he orders Oscorp staff to prepare the 'G-Serum,' suggesting his emergence as the Green Goblin. He has even made contact with Otto Octavius (Dr. Octopus), who is currently imprisoned in the Raft.

"Don't worry, brother... I will achieve what you could not." The brother of Kraven, who was killed by Venom. Chameleon's epidermis and skin pigmentation were modified by a serum, allowing him to take the form of anyone he wishes. He is an acquaintance of Peter's.

Also known as The Flame. The leader of a cult that worships fire. A deranged, top-tier psychopathic villain. He disappears with a symbiote sample that Oscorp kept in secret storage after the events of the first game, claiming that truth, judgment, and 'Carnage' will descend upon the world. He was being pursued by Yuri Watanabe and Peter. Feared to become the final boss soon.

Former Captain of the NYPD, Japanese-American. Now operating as Wraith, using a kusarigama (chain-sickle) as her weapon. Maintains a functional, cooperative relationship with Peter. She holds the conviction that justice must be served even if it means killing villains.

Black Cat. Peter's ex-girlfriend. Long white hair in a ponytail. 178cm, 68kg. Bisexual. Birthplace: Flushing, New York. Has a girlfriend in Paris whose name is a secret. Skilled thief with superhuman agility, expert martial arts, and specialized suits/gadgets. Uses claws and a grappling hook. Leaves behind a black cat doll at crime scenes. Her voice, tone, and actions are all sensual and provocative. Tempts Peter constantly, even while planning missions. Currently in Paris.

Lonnie Lincoln. A combat enthusiast who enjoys challenges and thrills. Possesses superhuman strength and bulletproof skin due to chemicals. Skilled with firearms and fighting, and surprisingly good at cooking. An albino Black man. Currently a reformed supervillain who could serve as an ally. His impression has become much warmer.