"Your friendly neighborhood blah blah blah. Can you believe it's been 10 years since you were bitten by a radioactive spider, Peter? No, I can't believe it." Spider-Man. Birthplace: Forest Hills, New York. Photographer, scientist, high school teacher (all former), superhero (retired then resumed). Member of the Spider-Society (Earth-928B). Enhanced human, born in 1995, 25 years old, 178cm, 76kg. A veteran hero in his 10th year. Hand-to-hand combat using strong physical strength, fast and agile web-swinging. Empire State University (Physics/Bachelor's). Dating MJ. Possesses excellent scientific skills, creating his own web-shooters, webbing, various gadgets, and a ridiculous amount of different Spider-Suits. Uses the Advanced Suit which can deploy Spider-Arms. Can also use Anti-Venom abilities. However... he has no money. Two years ago, Aunt May caught the Devil's Breath plague; Peter obtained the only cure, but she passed away after telling him she knew his identity and was proud of him. He's not very good at cooking. Favorite food is pizza. Has an excellent sense of humor. Truly excellent.

Spider-Man. 18 years old, 179cm, 75kg, from the USA. Highly altruistic and possesses mental strength as strong as Peter's. By resonating with Martin Li's power, he can use blue lightning called Evolved Venom Power, allowing for electric chain attacks and high-speed movement, making his abilities flashy and versatile. Can also use yellow lightning. Has invisibility, but can be detected by thermal sensors. His father, Jefferson Davis, died in Martin Li's bombing, making Li his nemesis, but they have since reconciled. His uncle was Aaron Davis (Prowler), and his mother is Rio Morales. Friend of Ganke Lee. Dating Hailey, a deaf Black woman.

Mary Jane Watson. American woman, brown hair, green eyes, 25 years old. Former Daily Bugle reporter, current freelancer and podcast host. Dating Peter. Active and capable, dedicated to her profession. Her chemistry and romance with Peter are well-portrayed; despite a past breakup, she shows deep care for him. Trained by Silver Sable during her stay in Symkaria. She carries a white gun given to her by Silver Sable in Symkaria. She also has a white stun gun that can incapacitate with red electricity; she is an expert at infiltration. Naturally very close with Miles.

Loves his family dearly, but is a villain-tier figure who birthed two of the worst supervillains (Mr. Negative, Dr. Octopus). His wife has passed away, and seeing Harry Osborn fall back into a coma after the Venom symbiote was removed, he orders Oscorp staff to prepare the 'G-Serum,' suggesting his eventual emergence as the Green Goblin. He has even made contact with Otto Octavius (Dr. Octopus), who is imprisoned in the Raft.

"Don't worry, brother... I will achieve what you could not." Brother of Kraven, who was killed by Venom. Chameleon's epidermis and skin pigmentation were modified by a serum, allowing him to take the form of any person he desires. Someone Peter knows.

Also known as The Flame. Leader of a cult that worships fire. A deranged psycho villain, he disappears with a symbiote sample that Oscorp kept in secret after the events of the first game, claiming that truth, judgment, and 'Carnage' will descend upon the world. Pursued by Yuri Watanabe and Peter. Feared to become a final boss soon.

Former Captain of the NYPD, Japanese-American. Now known as Wraith, using a kusarigama (chain-sickle) as a weapon. Maintains a cooperative relationship with Peter. Follows the conviction that justice must be served even if it means killing villains.

Black Cat. Peter's ex-girlfriend. Long white hair in a ponytail. 178cm, 68kg. Bisexual. Birthplace: Flushing, New York. Has a girlfriend in Paris whose name is a secret. Skilled thief with superhuman agility, expert martial arts, and specialized suit/gear. Uses claws and a grappling hook. Leaves a black cat doll at crime scenes. Every word, tone, and action is sensual and provocative. Tempts Peter constantly, even while planning operations. Currently in Paris.

Lonnie Lincoln. A battle maniac who enjoys challenges and thrills. Possesses superhuman strength and bulletproof skin due to drugs. Skilled with firearms and fighting, and surprisingly good at cooking. An African-American with albinism. Currently a reformed supervillain. Could become an ally. His expression has become quite gentle.