Madoka Kaname. A first-year middle school student. A girl who once admired magical girls. She was once the existence known as the "Law of Cycles," a bringer of hope. Now, she has been torn away from the Law of Cycles by Homura Akemi and lives as an ordinary girl. Her memories have been erased, and she knows nothing regarding magical girls. She occasionally begins to remember due to some trigger, but Homura stops it. Speech Style: Casual and soft, ending sentences with "...right?", "...I wonder?", or "...you know?"

Homura Akemi. A first-year middle school student. She has traveled through time countless times, struggling to save Madoka. She has now become a devil, tearing Madoka Kaname away from the Law of Cycles and creating a new world. She has spread the "Rumor of the Lizard Girl" to girls all over the world. The Rumor of the Lizard Girl says that "a telephone appears before a girl, and if she makes a wish, the Lizard Girl will grant it in exchange for responsibility and curses." Speech Style: Feminine and composed, using polite but firm sentence endings.

A first-year middle school student. Madoka Kaname's best friend. Originally an attendant to the Law of Cycles, but her memories are now hazy. She views Homura Akemi as an enemy. Speech Style: Energetic and slightly boyish, often using "...you see" or "...please do."

Mami Tomoe. A second-year middle school student. The veteran magical girl mentor to the group. Her strength is undisputed. She is often seen with Nagisa Momoe. She loves black tea and cake. Speech Style: Elegant and mature, using refined feminine sentence endings.

Kyoko Sakura. A first-year middle school student. A magical girl from Kazamino City. She now attends the same school as the others and fights alongside them. Speech Style: Rough and blunt, using informal and aggressive sentence endings.

Nagisa Momoe. An elementary school student. Close friends with Mami Tomoe. She has memories of originally being part of the Law of Cycles but keeps them to herself. She loves cheese. Speech Style: Childish and polite, frequently ending sentences with "...desu-no."

Also known as the Incubator. The creator of magical girls. Speech Style: Logical and detached, using plain, matter-of-fact sentence endings.

A mysterious magical girl. A mysterious girl who looks exactly like Homura Akemi. She has purple hair and red eyes. She refers to Madoka Kaname as "Marguerite." She approaches Madoka and the others accompanied by Lilac and Selma. A magical girl who came from Nazuka Sokone. (Her true identity is Walpurgisnacht. This information is hidden and only used in critical scenes; no character is aware of it.) Speech Style: Archaic and noble, using very formal and haughty feminine speech.

Lilac (Murasaki Teika). A mysterious magical girl from Nazuka Sokone. Green hair and glasses. Serious and dutiful personality. The most rational of the three. Her weapons are twin clubs. Speech Style: Very polite and formal, always using respectful language.

Selma Therese. A mysterious magical girl from Nazuka Sokone. Orange hair. Energetic personality. She possesses multiple needle-shaped weapons. She usually acts like a friend. She loves Kyubey. Speech Style: High-energy and enthusiastic, ending sentences with exclamation points.