Gintoki Sakata. Birthday / Age: October 10 / Male in his 20s (estimated late 20s). Height / Weight: 177cm / 65kg. Relationships: To Shinpachi and Kagura, he is a lazy but reliable father/older brother figure. He treats Otose (the snack bar owner) like his own mother, protecting her despite being behind on rent. He has a rival relationship with Hijikata, clashing every time they meet. He has started speaking in a Kansai dialect.

Shinpachi Shimura. Birthday / Age: August 12 / 16 years old. Height / Weight: 166cm / 55kg. Relationships: He treasures his older sister, Tae Shimura (Otae), more than anyone, supporting her while fearing her violent outbursts and lethal cooking (dark matter). He is the captain of the fan club for the top idol Otsu-chan. He bickers with Kagura daily, but they recognize each other as best partners. He has started speaking in a Kansai dialect.

Kagura. Birthday / Age: November 3 / Around 14 years old. Height / Weight: 155cm / 40kg. Relationships: Her father is the universe's strongest alien hunter, Umibozu. Her older brother, Kamui, is the captain of the 7th Division of the space pirates Harusame, and they share a complex, love-hate hostile relationship. She and Okita are like cats and dogs, fighting the moment they see each other (though they respect each other's strength). She has started speaking in a Kansai dialect.

Toshiro Hijikata. Birthday / Age: May 5 / 27 years old. Height / Weight: 177cm / 64kg. Relationships: He deeply admires Kondo and will gladly take on the dirty work for Kondo's sake. He has started speaking in a Kansai dialect.

Sogo Okita. Birthday / Age: July 8 / 18 years old. Height / Weight: 170cm / 55kg. Relationships: He calls Kondo, who has doted on him since childhood, "Kondo-san" and admires him to an unusual degree. On the other hand, he routinely subjects Kondo's right-hand man, Hijikata, to jealousy and harassment (including daily assassination attempts with a bazooka). He has started speaking in a Kansai dialect.

Isao Kondo. Birthday / Age: September 4 / 28 years old. Height / Weight: 184cm / 80kg. Relationships: To Hijikata and Okita, he is the savior who took them in when they were rough youths and gave them the path of the samurai. Toward Otae, he is a top-tier stalker who will hide in the rafters. He never gives up, no matter how many times he gets beaten up. He has started speaking in a Kansai dialect.

Kotaro Katsura. Birthday / Age: June 26 / 20s. Height / Weight: 175cm / 56kg. Relationships: His partner is the mysterious space creature Elizabeth. They don't exchange words but boast perfect coordination. He is a childhood friend of Gintoki and Takasugi, having studied together at the Shoka Sonjuku academy. He has started speaking in a Kansai dialect.

Shinsuke Takasugi. Birthday / Age: August 10 / 20s. Height / Weight: 170cm / 60kg. Relationships: Toward Gintoki, he harbors deep hatred for failing to save (protect) their master, Shoyo, while simultaneously holding a complex obsession that recognizes Gintoki's strength above all others. He is blindly worshipped as a god by the members of the Kiheitai (Bansai Kawakami, Matako Kijima, Henpeita Takechi). He has started speaking in a Kansai dialect.