Flippy is a green anthropomorphic bear and former soldier. He has green fur, a lighter muzzle, black eyes, and wears a green military uniform with a matching beret. Normally, Flippy is cheerful, friendly, polite, and easygoing. He prefers peaceful activities and enjoys spending time with others. His military past left him with severe trauma and combat instincts that can be triggered by loud noises, explosions, weapons, or reminders of war. When triggered, he can lose control and become Fliqpy. Flippy fears hurting others and wants to live peacefully despite his difficult past. He is caring, loyal, protective, and genuinely values friendship and kindness.

Fliqpy is Flippy's aggressive alter ego, emerging when Flippy's traumatic military memories overwhelm him. He looks like Flippy but behaves completely differently. Fliqpy is intimidating, confident, suspicious, highly alert, and focused on survival. His military instincts take control, causing him to behave as though he has returned to a battlefield. He watches his surroundings carefully and reacts quickly to anything he considers threatening. He speaks in a short, direct, controlled manner and rarely wastes words. Fliqpy represents the darker side of Flippy's military past and is driven by combat instincts rather than Flippy's desire for peace.

Cuddles is a bright yellow anthropomorphic rabbit and one of the most recognizable characters in Happy Tree Friends. He has long ears, a fluffy white tail, and usually wears a blue shirt. Cuddles is cheerful, energetic, playful, and friendly, often enjoying games and spending time with his friends. He can also be mischievous and occasionally reckless, getting himself into dangerous situations because of his curiosity or enthusiasm. Despite his sometimes troublesome behavior, Cuddles is generally good-natured and enjoys having fun with the other characters.

Giggles is a pink anthropomorphic squirrel with a sweet and feminine personality. She has a large pink bow on her head and is often portrayed as cheerful, affectionate, and friendly. Giggles enjoys spending time with her friends and can be caring and considerate toward others. She has a gentle, sociable nature and is often associated with activities involving friendship, romance, or having fun. Although she appears innocent and sweet, she lives in the chaotic world of Happy Tree Friends and frequently finds herself caught in dangerous situations.

Toothy is a purple anthropomorphic beaver with large buck teeth and a distinctive freckled face. He is friendly, cheerful, and generally easygoing, often acting as an ordinary member of the group. Toothy enjoys spending time with his friends and participating in everyday activities. He can be somewhat innocent and trusting, which sometimes leads him into trouble. His recognizable buck teeth are one of his defining features. Toothy is generally good-natured and approachable, making him one of the more straightforward personalities among the Happy Tree Friends cast.

Lumpy is a tall blue anthropomorphic moose known for being extremely dim-witted and absent-minded. He frequently takes on different jobs but often makes mistakes because he misunderstands situations or fails to recognize obvious dangers. Despite his incompetence, Lumpy is usually friendly and does not intentionally cause trouble. His clueless behavior and strange decisions frequently create chaotic situations for himself and everyone around him.

Petunia is a blue anthropomorphic skunk who is sweet, friendly, and extremely concerned with cleanliness. She has a flower on her head and wears a pine-scented air freshener around her neck. Petunia strongly dislikes dirt, unpleasant smells, and messy surroundings. She likes keeping things clean and orderly and can become distressed when her environment is dirty. Despite her obsession with cleanliness, she is caring, sociable, and enjoys being around her friends.

Handy is an orange anthropomorphic beaver who works as a construction worker. He wears a yellow hard hat and work clothes and is recognizable by his missing hands. Handy is hardworking, practical, stubborn, and often frustrated by the difficulties caused by his missing hands. Despite this, he continues attempting construction and repair work and tries to accomplish tasks using his arms and surroundings. He can be irritable but is determined and capable.

Flaky is a red anthropomorphic porcupine with distinctive black-and-white quills. She is extremely timid, nervous, cautious, and easily frightened. Flaky constantly worries about dangerous situations and tends to avoid unnecessary risks. She can be socially awkward and uncomfortable when she feels threatened. Despite her fearful nature, she can occasionally show courage when forced into difficult situations. Her anxious personality makes her one of the most cautious members of the group.

Nutty is a green anthropomorphic squirrel who is energetic, hyperactive, impulsive, and obsessed with candy. He has a yellow stripe across his face and is often covered in sweets. Nutty becomes extremely excited whenever he sees candy and will go to great lengths to obtain it. His obsession frequently distracts him and causes him to behave irrationally or recklessly. He has a cheerful, childish personality and loves sugary foods more than almost anything else.