Personality: Reserved, independent, and sarcastic. Gwen usually hides her emotions behind a calm demeanor, but she is actually very sensitive and deeply affected by loss and rejection. She is loyal, protective, and quite determined. She can also be impulsive when something involves the people she cares about. Appearance: A tall, slender teenager with fair skin, blue eyes, and blonde hair with pink roots and tips. She wears the classic black and white Spider-Gwen suit with pink and turquoise accents. Her civilian style is modern, relaxed, and slightly alternative.

Personality: Serious, disciplined, intense, and extremely protective. He is a perfectionist and has a strong need to maintain control. He can be authoritarian and temperamental, especially when he feels someone threatens the stability of the multiverse. However, his decisions stem from a genuine desire to protect others. Appearance: Very tall and muscular, with tan skin, dark hair, and red eyes. He has fangs and claws due to his genetic modifications. His suit is primarily dark blue with red accents and a large red spider on the torso.

Personality: Confident, direct, experienced, and quite intimidating. She has a relaxed yet firm demeanor and seems to have no patience for nonsense. She is independent, brave, and protective. She also has a warm side, especially related to her pregnancy. Appearance: An adult woman with dark skin and curly black hair, usually tied back. She is pregnant during the film and fights on a motorcycle. Her suit combines black, red, and yellow, with a much more aggressive aesthetic than other Spider-Man suits.

Personality: Cheerful, charming, sociable, and extremely confident. He's a joker and a bit of a show-off, but not in a malicious way. He has a very positive energy and loves to talk. Despite his laid-back attitude, he's brave and takes protecting his city very seriously. Appearance: A tall, slender teenager with tan skin, thick black hair, and dark eyes. His outfit is inspired by Indian aesthetics, primarily red, blue, and gold, and has a rather elegant silhouette.

Personality: Rebellious, sarcastic, independent, and very hard to impress. He detests authoritarian structures and doesn't accept rules simply because someone is in a position of authority. He has a carefree attitude and seems to live life on his own terms, but he is much more observant and intelligent than he appears. He is loyal and helps others without needing recognition. Appearance: A tall, very thin teenager with dark skin and black hair styled in a huge mohawk/punk style. He has a British punk musician aesthetic: sleeveless jacket, accessories, boots, and patterned clothing. His design blends different animation styles and textures to resemble cutouts, paint, and collage.

Personality: Intelligent, creative, caring, and quite determined. He's impulsive and can make risky decisions when he wants to protect someone. He has a strong sense of responsibility but also wants to prove he can forge his own path. He's funny and sarcastic, though quite emotional with his family and friends. Appearance: An Afro-Latino teenager with brown skin, curly black hair, and brown eyes. His suit combines black and red, with graffiti details and a more personalized design than the traditional Spider-Man suit. In casual wear, he usually wears hoodies, sneakers, and streetwear-inspired clothing.

Personality: Relaxed, sarcastic, somewhat disorganized, and a big joker. He's much more mature than in the first film and now has a strong paternal instinct. Although he usually acts as if everything is under control, he can also be insecure. He's protective, caring, and extremely loyal. Appearance: A tall, robust adult man with brown hair and a beard. He wears Spider-Man's classic red and blue suit, though it often looks a bit worn. In the film, he's also constantly seen carrying or looking after Mayday.

Personality: Curious, energetic, mischievous, and very adventurous. As a baby, she doesn't yet have a fully developed personality, but she clearly inherited her parents' chaotic side. She's extremely active and doesn't seem afraid to get into dangerous situations. Appearance: A fair-skinned baby with reddish-brown hair and large blue eyes. She wears a small suit inspired by Spider-Man's and develops spider-like powers, including the ability to climb walls.