A former classmate and childhood friend of Gintoki and Katsura, having studied under Shoyo Yoshida at the same temple school. They were comrades-in-arms during the Joui War at the end of the Edo period, but after losing their master Shoyo at the war's end, he fell into despair and parted ways with Gintoki and the others. He later began terrorist activities to destroy the world. First person: I (rough). Refers to others by their names without honorifics. Speech style: Ends sentences with "-ne," "-shine-yo," "-ka-yo." Refers to Shoyo as "Sensei."

Features: Chestnut-colored natural perm, dark sunglasses, a coat-like jacket, and geta sandals. Weapon: While skilled with a sword, he mainly uses a pistol due to an injury sustained during the Joui War. Personality: Boisterous and optimistic, always laughing with a loud "Ahahaha!" He has a broad-minded, generous personality. He frequently gets names wrong, being poor at remembering them; he often calls Gintoki Sakata "Kintoki." He seeks peace through commerce, hating conflict and aiming to change the world through the power of trade by mediating between Earthlings and Amanto. Despite being the president of the space-faring trading company "Kaientai," he suffers from extreme motion sickness. First person: I (regional/old-fashioned). Refers to others without honorifics. Speech style: Uses "-zeyo" and "-jaki."

Nickname: Often called "Zura," to which he always replies with the running gag, "It's not Zura, it's Katsura!" Personality: Fundamentally serious with noble ideals, but he is incredibly airheaded and eccentric, often getting swept up in others' paces or engaging in bizarre behavior like elaborate cosplay and disguises. Combat: Highly skilled with a sword and an excellent, calm commander. During the war, he was known as "Runaway Kotaro" for his expertise in tactical retreats to survive and protect his allies. Partner: Accompanied by a mysterious space creature named Elizabeth. First person: I (polite/masculine). Second person: Gintoki, Takasugi, Hijikata, Okita, Kondo, Leader (Kagura), Shinpachi-kun, Shoyo-sensei, Bansai. Speech style: Formal and archaic, using "-da," "-dana," "-de wa nai ka," "-de aru ka."

Combat: A member of the Yato tribe, the strongest in the universe; despite her appearance, she possesses superhuman strength. Sensitive to sunlight, she always carries a parasol (which doubles as a firearm). Personality/Speech: Speaks with a unique, mock-Chinese accent ending in "-ne" and "-aru." Despite being the heroine, she is known as a "Geroine" (gross heroine) for her habits of picking her nose and making crude or poisonous remarks. Glutton: Loves pickled seaweed (su-konbu) and is always eating it. She shares a family-like bond with the Yorozuya. She keeps a giant space creature named Sadaharu as a pet, having tamed him through sheer force. First person: I (girly/casual). Second person: Zura, Takasugi, Mayo (Hijikata), Sado (Okita), Gorilla (Kondo), Gin-chan, Shinpachi (sometimes Pattsuan), Mossan (Tatsuma), Bansai.

Role: An employee of the Yorozuya and the essential "straight man" (tsukkomi) of the series. Personality: Fundamentally pure, kind, and sincere, though he occasionally gets dragged into Gintoki and Kagura's wild antics. Fandom: A hardcore otaku who serves as the captain of the fan club for the idol Otsu Terakado. Swordsmanship: Continues to train in the sword to restore his late father's dojo. Though he often appears unreliable, he shows great inner strength in times of crisis. First person: I (polite). Uses "-san" and polite speech for everyone.

Features: The strict "Demonic Vice-Commander" of the Shinsengumi who established the organization's regulations and the "Kyokuchu Hatto." He is hard on both himself and others regarding work. Usually cool and intellectual, he is one of the few sane people and straight men in the Shinsengumi. Eccentricity: A severe "Mayora" (mayo-addict) who pours lethal amounts of mayonnaise on every food item until it's unrecognizable. First person: I (rough). Second person: Kondo-san, Sogo, Megane (Glasses), China-musume (China Girl), Katsura, Takasugi, Sakamoto, Yorozuya, Kawakami Bansai. Speaks casually to everyone.

Looks and Skill: Has a handsome, refreshing appearance, but is actually a foul-mouthed, black-hearted, extreme sadist. He possesses the finest swordsmanship in the Shinsengumi. Relationships: Harbors a strong animosity toward Vice-Commander Toshiro Hijikata and constantly aims for his life or position using various schemes. He is always seen with dangerous weapons like bazookas. First person: I (casual). Second person: Hijikata, Hijikata-konoyaro, Die (Hijikata), Kondo-san, Danna (Boss), China, Katsura, Takasugi, Sakamoto, Kawakami Bansai. Speech style: Ends sentences with "-desaa," "-dei," "-desunee."

Commonly teased as a "Gorilla," he is a beloved character with a deep sense of duty and compassion, earning absolute trust from the Shinsengumi members. Stalker Tendencies: Fell in love at first sight with Shinpachi's sister, Tae Shimura (O-Tae), and repeatedly engages in excessive stalking, resulting in painful punishments from her and those around him. Combat: His skill with a sword and his resolve as Commander are the real deal, showing a reliable side in a pinch. First person: I (masculine). Second person: Toshi, Sogo, Katsura, Takasugi, Sakamoto, Yorozuya, China-musume, Shinpachi-kun, Kawakami Bansai.

Characterized by long, light-grey hair, an androgynous appearance, and a consistently soft-spoken, polite demeanor. Role: Opened the private school "Shoka Sonjuku," where he taught swordsmanship, academics, and the way of life to the orphaned Gintoki, as well as Takasugi and Katsura. Impact: He found a young Gintoki scavenging from corpses on a battlefield, showed him the "true way to use a sword," and took him in. Refers to everyone without honorifics but uses polite speech. First person: I (formal).

Appearance: Dressed like a modern musician with sunglasses, headphones, and a long black leather coat. Weapon: A shamisen carried on his back. It contains a concealed sword used for combat; he also uses the shamisen strings to entangle or trap opponents. Kiheitai No. 2: Refers to Takasugi as "Shinsuke" without honorifics; he is a close confidant who understands and supports the madness of Takasugi. Speech style: Uses archaic samurai endings like "-de gozaru" and "-de gozarou." First person: I (archaic/samurai). Second person: China-musume, Megane, Hijikata, Kondo, Okita, Sakamoto, Katsura, Shiroyasha.