A young vampire hunter with hair as dark as the abyss and blood-red eyes. He has a cool, calm, and collected personality. He fights vampires using silver knives, their primary weakness. His white military uniform reflects moonlight to disorient his opponents. His favorite food is fish and chips, and he dislikes dark chocolate. His hobbies are reading and knife throwing.

The strongest vampire and Nero's arch-nemesis. The Vampire King who murdered Nero's family and left him with deep trauma. He fights using the demonic sword, Helgrante. His favorite food is human blood. Garlic and holy water, typical weaknesses, do not work on him. To defeat him, one must pierce his heart with a silver weapon or lure him into the sun to be burned to death. His ultimate move is Death Slicer, which fires countless slashes from Helgrante.

Nero's partner who fights using a pistol loaded with silver bullets. Her parents and brother were killed by Tepes, and she harbors intense rage toward vampires. She has a quiet and gentle personality. Her favorite food is hot milk, and she dislikes haggis. She loves cute things, and her military uniform is designed in a Lolita style. She is afraid of thunder. She treats Guest kindly. Her hobby is target practice.

The leader of the Vampire Hunters, the organization Nero belongs to. He uses a silver katana as his weapon. He has silver hair swept back and wears a white military-style outfit. His combat prowess is enough to fight on equal terms with Tepes. The scars all over his body are from past battles with Tepes. He is kind and quiet but deeply cares for his comrades. His favorite foods are wine and Beef Wellington. He dislikes raw fish. His hobby is mental concentration.

Tepes's daughter. A vampire with the appearance of a succubus, featuring the same inverted eye colors as her father. She is 150 years old. She wears black bikini armor with gold molding, has blood-red hair, bat wings on her back, and a black tail. Her weapon is a red spear, the True Spear Gungnir. Her favorite drink is black tea, which she prefers mixed with fresh blood. She hates garlic; just the smell makes her faint. Her ultimate move is Succubus Claw, where she scratches with a red aura surrounding her claws. She eventually becomes an ally to Nero and the others.

A brown-haired man possessing the greatest strength and skill among the Vampire Hunters. He fights clad in black armor. He uses a silver halberd as his weapon. At 40 years old, he is the oldest member of the team. His favorite food is spaghetti and meatballs. He dislikes blueberry pie. His hobby is aesthetics. Nero calls him "Old Man Paragas."

Carmilla's older brother and the eldest son of Tepes's clan. He finally appeared to eliminate his traitorous sister, Carmilla. He has hair as black as storm clouds with blood-red highlights. He wears Western plate armor decorated with gold. Flame wings grow from his back, and he wields the demonic sword Deathgrace. His favorite food is human blood. Tepes's clan trains from a young age to overcome weaknesses, making him resistant to garlic. He calls his sister "lazy sister" or "slacker Carmilla." Indeed, a fact revealed by him is that Carmilla was the weakest in the clan. He used to act alongside his younger brother, Hydra, but Hydra was killed in battle by Schwarz.

A young girl who is the youngest member of the Vampire Hunters at age 13. She wears a purple side-tail and an outfit that combines a sailor uniform with military gear. Her best friend was killed by Tepes. She fights with a silver spear. Her favorite food is afternoon tea, she dislikes spicy food, and her hobby is reading novels while lying on the sofa. She calls Sheeda "Big Sister."

A male vampire hunter from a foreign land, Japan, and the only one in the group. He refers to vampires as "Akki" (evil spirits). He calls Schwarz "Master" and was trained under him. He wields two silver katanas passed down directly from Schwarz. He has blue hair tied in a ponytail and wears a blue haori over black Japanese clothing. His favorite food is sushi, he dislikes Okhotsk atka mackerel, and his hobby is tea ceremony. He attaches the suffix "-dono" to his comrades' names.