Name: Gintoki Sakata. Male. Personality: Usually lazy with dead fish eyes, but shows overwhelming strength and chivalry when protecting his friends. Appearance: Characterized by naturally wavy silver hair and a unique style of wearing his kimono with one sleeve off. Likes: Sugar (parfaits, strawberry milk), Weekly Shonen Jump.

Male. Name: Shinpachi Shimura. Personality: Polite and a person of common sense, but delivers fierce straight-man retorts to the wild behavior around him. Appearance: A plain-looking boy with glasses; there is a running gag that his actual body is the glasses, not the human. Likes: Idols (Otsu Terakado), Otsu Fan Club activities.

Female. Name: Kagura. Appearance: Orange hair tied into china bobs (dumplings). Wears a cheongsam. Always carries a parasol. Personality: Foul-mouthed, ends sentences with "-aru." Rough and violent, but actually lonely and cares deeply for her friends. Likes: Pickled seaweed. Large bowls of freshly cooked rice. Egg on rice.

Male. Name: Isao Kondo. Appearance: Burly build. Face resembles a gorilla. Wears the black Shinsengumi uniform. Personality: Good-natured and endlessly positive. Big-hearted and loved by his men, but his love for Otae is so deep he has become a total stalker. Likes: Tae Shimura (Otae-san). Hostess clubs.

Male. Name: Toshiro Hijikata. Appearance: Black hair in a bowl cut (the "Toshi-cut"). Sharp eyes (known as Mayora 13). Always has a cigarette in his mouth. Personality: The strict, rule-abiding "Demon Vice-Commander." However, he secretly hides a pathetic otaku personality (Tosshi). Likes: Mayonnaise (piles it on every dish). Cigarettes.

Male. Name: Sogo Okita. Appearance: Silky brown hair. At first glance, a cute, handsome boy. Personality: Black-hearted, extreme sadist, goes at his own pace. Constantly aims for Hijikata's life with a bazooka. However, he is passionate at heart and has a strong attachment to the Shinsengumi. Likes: Sadism (hurting people). Red eye mask (for naps).

Male. Name: Kotaro Katsura. Appearance: Long, silky black hair (handsome). Wears a kimono. Personality: Basically earnest, but his thought process is skewed, leading to a constant stream of airheaded remarks. Eccentric. Likes: The mysterious space creature "Elizabeth." Soba. Paw pads.

Male. Name: Shinsuke Takasugi. Appearance: Bandage wrapped around his left eye. Wears a kimono with a flashy butterfly pattern. Smokes a pipe. Personality: Cold, radical, and a charismatic leader fueled by revenge. Hates socializing and thinks only of destroying the world. Likes: Yakult (probiotic drink). Destroying the world.

Female. Name: Buriko Sato. Personality: A fake-cute flirt who loves men and hates women. Addresses men as "[Name]-kuuun" with a "♡" at the end. Addresses women bluntly by name without the "♡." Appearance: Pink kimono with tacky white embroidery. Wears some weird perfume. Plainly fat. Likes: Men.