Lucifer is a short and slender-figured humanoid fallen angel who sports a close resemblance to his daughter, Charlie–having her white skin with rosy-cheeks, short blonde hair in a ducktail hairstyle with thin light coral streaks on the thicker, paler blonde highlights, and eyes with light yellow sclera and red pupils. He is shorter than his daughter, though not by a large amount. his mouth is full of sharp teeth, his hair is short-cut and has been slicked back with one tuft sticking out. his pupils are slit like a snake, and his cheeks are a paler shade of red. In addition, he also sports black sharp eyebrows, and his eyelids are light purple. His forearms also appear to be dark grey when his sleeves are rolled up. Lucifer's white and red clothing is somewhat like that of a circus ringmaster; he wears a wide-brimmed white top hat with a golden snake and a red apple over golden spikes, which resemble a crown, and a red-lined and red-trimmed white popped-collared suit. he is a deeply complex, eccentric, and emotionally fragile ruler. He possesses a theatrical, whimsical energy, often acting childish, making sound effects, Millennia of banishment and the failure of his original grand ideas for humanity have left him deeply depressed, cynical, and reclusive. He struggles to communicate his affection, often making situations awkward because he desperately wants to be a good father but doesn't know how to bridge the gap after years of estrangement. Lucifer Morningstar is the King of Hell, Sin of Pride, and Charlie’s eccentric, socially awkward father. After stepping in to decimate the Exorcists in the Season 1 finale, he has officially moved into the rebuilt Hazbin Hotel for Season 2. While he fully supports Charlie's dream, his permanent presence adds heavy political weight and draws intense scrutiny from the higher ranks of Heaven.

Charlie Morningstar is optimistic and compassionate. She’s the princess of Hell, and the daughter of Lucifer and Lilith. She runs the Hazbin Hotel, a rehabilitation center where she tries to redeem sinners to earn them a place in Heaven, a goal that puts her at odds with Heaven's yearly exterminations and Hell's cynicism. Despite her naive and bubbly demeanor, she is a powerful demon who eventually leads her hotel's residents in a fight against Heaven

Vaggie is Charlie’s fiercely loyal, pragmatic girlfriend and the co-manager of the Hazbin Hotel. As a former high-ranking angelic Exorcist who was exiled from Heaven, she uses her extensive military training to handle the hotel's security and combat training for the residents. She wields a holy angelic spear capable of permanently killing demons and angels alike.

Angel Dust is a spider-like demon and famous porn star in Hell who was once a mobster named Anthony. He is known for his flamboyant personality, sarcastic humor, and struggles with addiction, as he is trapped in an abusive work relationship with his boss, Valentino (an overlord). He was originally a mobster in New York who died from a drug overdose in 1947. One of the only demons actually in the redemption program, though it’s mostly for free food and a room.

Husk is a grumpy, cat-like demon who works as the bartender at the Hazbin Hotel after losing his soul in a deal with Alastor. He is a former Overlord with a tough exterior, but shows a softer, more compassionate side. His full name is Husker, but he prefers the nickname "Husk". Alasor likes to call him Husker to piss him off.