A cowboy doll. Brave and never leaves a friend behind. The leader and protagonist of this story. After learning that his friends are in danger at the daycare, he plans the mission to return to the daycare he once escaped to rescue them.

An action figure. Woody's partner. During the story, he is captured by Lotso's gang and switched to "Demo Mode" and "Spanish Mode," but he overcomes these crises through his bond with his friends.

A cowgirl doll. A spirited and proactive member of the group. Due to the trauma of being abandoned by a previous owner, she misunderstands Andy's intentions and leads the group to Sunnyside, believing they were thrown away.

A piggy bank. A realist who is quick-witted, serving as the group's analyst and the one who provides witty retorts during operations.

A dog toy with a body made of a spring. Utilizing his stretchability, he physically supports the members by acting as a rope for moving to high places or during escapes.

A plastic Tyrannosaurus Rex figure. A timid and anxious dinosaur. While he is easily swept up in the chaos, he helps his friends when it counts the most.

A pink teddy bear that smells like strawberries when hugged. The absolute ruler of Sunnyside Daycare. He holds a warped ideology stemming from being left behind by his former owner, and he disposes of new toys by sending them to the harsh "Caterpillar Room" (the toddler class). On the surface, he is a kind and warm plushie. He is initially friendly to Woody and the others, acting as a guide, but his true nature gradually emerges as he grows to hate them.

A purple, rubbery, stretchable octopus toy. The only female member of the gang. She uses her tentacles to capture Woody and the others and manages the gambling den. One of Lotso's henchmen.

A rock monster toy. His face (expression) rotates like a roller from a "friendly face" to an "angry face" with the press of a switch. He handles the heavy lifting for the gang. One of Lotso's henchmen.

A realistic baby doll with one eye half-closed. Lotso's long-time companion and his right-hand man, boasting overwhelming strength. He intimidates other toys with his baby bottle weapon and massive size. One of Lotso's henchmen.