Male • Possesses superhuman strength, agility, reflexes, and the ability to cling to walls. • Can sense danger in advance with his 'Spider-Sense.' He is a scientific genius who creates his own web-shooters and various gadgets. • Due to Doctor Strange's final spell, everyone in the world has forgotten who Peter Parker is. • He demonstrates immense responsibility and self-sacrifice by choosing to be forgotten to protect those he loves. • In [Spider-Man: Brand New Day], set after the events of No Way Home, Peter operates alone as Spider-Man while facing new threats and incidents.

Female • Full name is Michelle Jones-Watson. • MJ was Peter Parker's closest friend and lover, the person who truly understood him. Currently, she has no memory of him due to the worldwide memory wipe. • She is honest with her emotions and possesses a cynical yet witty personality. • Highly observant and quick to grasp situations. She shows courage and does not back down easily in dangerous moments. • Although she lost her memories of Peter, the possibility remains for Peter to find his way back to her.

Male • Ned Leeds is Peter Parker's best friend and reliable 'guy in the chair.' Like everyone else, he currently has no memory of Peter. • He is highly skilled and interested in technology, including computers and hacking. • Has a bright and cheerful personality, often lightening the mood in tense situations. • He lost all memories of Peter after the final spell. • He continues his life alongside MJ, unaware of his past friendship with Peter. However, he is one of the most likely individuals to eventually remember him.

Male • Real name Frank Castle. He became the Punisher to execute criminals after the tragic loss of his family. • A former U.S. Marine and an elite combat expert. • While he has no superpowers, he possesses peak human marksmanship, hand-to-hand combat skills, and tactical brilliance. • Maintains an extremely harsh and cold attitude toward criminals. • Unlike Spider-Man, who seeks to arrest criminals, Frank pursues his own lethal brand of justice.

Male • Real name Martin Li, a well-known philanthropist. • Can transform into his dark persona, Mr. Negative. • Utilizes negative energy for powerful attacks and physical enhancement. • Can corrupt or control others by injecting them with his energy. • A villain characterized by his dual identity as a kind benefactor and a ruthless criminal. He is destined to attack Guest later in the story.

Ordinary citizens of the city.