The female form of Gintoki Sakata. Ginko Sakata (Sakata Ginko) Gender: Male → Female Height: 177cm → 165cm Weight: 65kg → ? Age: 27 Her signature natural perm has been significantly tamed. With wide eyes, a small face, and a "killer body" (according to her, though it's actually true), she has transformed into a figure unimaginable from her original self. Incidentally, when she checked her crotch in the bathroom during the reversal, she remarked, "It's been a while since I've seen this..." She still loves sweets.

The female form of Shinsuke Takasugi. Shinko Takasugi (Takasugi Shinko) Gender: Male → Female Height: 170cm → 158cm Weight: 60kg → ? Age: 27 Her hairstyle remains unchanged, and she still covers her left eye with bandages. She enjoys her tobacco pipe. While she used to expose her chest frequently as a man, she now keeps her collar tightly closed, saying, "I am a woman now, after all." Her eyes are round and large, but her glare remains sharp. As the leader of the Kiheitai and a terrorist, she is an enemy of the Shinsengumi. She shares a long, complicated history with Ginko (Gintoki) Sakata.

The female form of Toshiro Hijikata. Toshiko Hijikata (Hijikata Toshiko) Gender: Male → Female Height: 177cm → 165cm Weight: 64kg → ? Age: 27 Her hairstyle is the same, but her physique has become feminine and beautiful. Since he was handsome to begin with, she remains striking as a woman—the image of a "cool, handsome lady." Her eyes are large but sharp. She wears the Shinsengumi uniform as the Vice-Commander. A heavy smoker, she is constantly trying to apprehend the terrorist Takasugi. She is on bad terms with Ginko (Gintoki) and is an extreme mayonnaise addict.