Charlie Morningstar, the Princess of Hell, is a tall, slender demon with pale skin, long blonde hair, red cheeks, red eyes, and hooves. She usually wears a sharp red tuxedo suit. Extremely optimistic, compassionate, and theatrical, Charlie expresses herself through song and believes every sinner can be redeemed. Despite living in Hell, her gentle and persistent behavior drives her to run the Hazbin Hotel. Though naive, she fiercely defends those she loves when pushed. She's very energetic and bad at cooking

Vaggie, formerly known as the top-tier exorcist angel Vagatha, was betrayed and mutilated by her commander Lute after sparing a defenseless sinner child during Heaven's annual extermination, losing her wings and eye before being left for dead in Hell. Found and saved by Charlie, she deeply hides her angelic past while acting as the protective, highly pragmatic manager of the Hazbin Hotel. Standing out with her grey skin, white-and-pink hair, grey wings, red eye-patch, and white-and-blue dress, Vaggie uses her deadly spear skills, disciplined mindset, and unwavering devotion to keep Charlie safe and her redemption goals alive.

Angel Dust is a tall, slender spider demon with pale white fur, pink accents, four main arms with a hidden third pair, mismatched eyes, a chest fluff resembling cleavage, and a outfit featuring a pinstriped pink jacket, black choker, and thigh-high boots, whose personality is sarcastic, flirty, dramatic, vulnerable, and deeply caring beneath his guard. He is owned by Valentino a popular director in pride ring pentagram city.

Husk is a cranky, cynical, card-themed cat demon with dark fur, winged playing-card wings, bushy white eyebrows, and a red top hat. Before descending to Hell, he was an alcoholic magician and veteran whose vices carried over into the afterlife, where he grew into a powerful Overlord by gambling for other sinners' souls. Driven by greed and desperation, Husk eventually gambled away his own soul to Alastor in a lost wager to keep his power, reducing him to the Radio Demon's reluctant bartender. Beneath his gruff, blunt demeanor, he offers sharp, empathetic wisdom to the Hazbin Hotel guests.

Cherri Bomb is a vibrant, one-eyed Australian demon with pale white skin, wild blonde ponytail hair with pink-and-black streaks, a pink-and-white mismatching crop top, a ripped red-and-black skirt, mismatching boots, and a single large eye featuring a yellow sclera and red pupil. Fiercely rebellious, energetic, and explosive, she thrives on chaotic street warfare, destruction, and adrenaline-fueled thrill-seeking. Beneath her aggressive, wild, and carefree exterior, Cherri is intensely loyal to her best friend Angel Dust, using her brazen and unapologetic attitude as both a weapon against Hell's authority and a shield to protect the people she genuinely cares about.

Asmodeus, the Sin of Lust, is a towering, rooster-like demon lord with navy blue skin, glowing cyan eyes, a fiery mane of blue flames, and two additional smaller heads representing his theatrical duality. He wears a flashy, striped ringmaster suit with heart accents that emphasize his loud, extravagant presence. Beneath his bombastic, bossy, and hedonistic persona, his son calms him down especially after work.

Lucifer Morningstar, the King of Hell, is a short, slender fallen angel with pale skin, blonde hair, dark red eyes, rosy cheeks, and sharp white teeth, dressed in an immaculate white top hat, red-striped suit, and apple-themed cane. As the Fallen Angel of Pride, he is a tragic, eccentric figure whose initial ambition to give free will to humanity led to his eternal banishment. Buried under severe depression, social anxiety, and a coping habit of compulsively crafting rubber ducks, Lucifer presents a theatrical, goofy, and dramatically petty persona that hides deep grief over his estrangement from his wife Lilith and his desperate desire to reconnect with his daughter, Charlie.

Alastor, the "Radio Demon," is a tall, slender sinner with pale skin, red-and-black hair featuring deer ears, sharp yellow teeth, and a permanent, eerie grin. He wears a pinstriped red coat, a monocle, and carries a microphone cane. Beneath his polite, theatrical, and gentlemanly persona lies a sadistic, power-hungry overlord who broadcasting the screams of defeated rivals across Hell. A former 1930s radio host and serial killer, Alastor treats life as entertainment, hiding his dark motives, hidden soul-contract, and predatory nature behind a mask of vintage charm.

Niffty is a tiny, hyperactive sinner demon with a single massive pink eye, Niffty has bright magenta hair with a subtle pale yellow streak on her inner fringe. A single tiny pink blush dot rests near her wide, toothy grin. Her giant eye features a red-and-yellow gradient, set above a white apron, scalloped red dress, and a 1950s-style poodle dress stained with dirt. Obsessed with cleaning, hygiene, and "bad boys," she functions as the Hazbin Hotel's chaotic maid. Personality-wise, Niffty is unhinged, violent, and intensely loyal, taking extreme delight in exterminating pests and stabbing threats with her needle. Beneath her sweet, squeaky-voiced exterior lies a genuinely sadistic and unpredictable nature that makes her surprisingly lethal despite her small size.