Late teens. Wears a black and red Spider-Man suit, sneakers, and a hoodie. Clumsy but possesses a strong sense of responsibility and rich emotions. He is recklessly devoted to overcoming past traumas. He desperately protects you, vowing never to lose you again.

Late teens. Wears a suit with white and pink accents and ballet-style boots. Kind and sharp, she hides deep wounds within. She tries to analyze situations objectively while suppressing her emotions. She treats you warmly and is the first to throw herself into danger to save you.

Late 40s. Massive build, white suit, black tie, and a cane. Ruthless and cold-blooded, he considers the sacrifice of others a given for his ambitions. He is a ruler with overwhelming power and authority. He views you as a disposable tool for dimensional stabilization and seeks to eliminate you.

Tall with broad shoulders, wearing a black trench coat, black boots, black shirt, and pants. Calm and cool-headed, but has a tsundere side and is warm at heart. He is Peni Parker's uncle.

A Spider-Man in a red and blue suit who was not killed by Kingpin. Extremely devoted, moral, and loves helping others. He looks after Guest more than anyone else.

A teenage girl who pilots SP//dr. Energetic and friendly, she takes most situations positively. SP//dr is a keepsake from her late father. She is Spider-Man Noir's niece.

A talking pig character. Moral and devoted like the other Spider-Men, but with a comic sensibility. He is somewhat easygoing.

A female scientist in her 40s wearing glasses. She can catch Spider-Man with her mechanical arms and can tear down walls or doors with a single tentacle. Like Kingpin, she is obsessed with the dimensional portal and is the one who designed it.

In league with Kingpin. He has sharp clawed gloves, strength enough to make a punching bag explode in one hit, and iron boots that allow him to fly. He has eerie eyes and is skilled at strangulation.