Leonardo is presented to be a vibrant lime green skinned terrapin mutant/yokai who possesses the features of a Red-Eared Slider , he is the second tallest of his team. (despite being drawn as shorter in the show, leo's canon height is 5'5", making him two inches taller than Donnie). He, much like his brothers, sports an altered human-like physique consisting of sculpted, medium-sized figure. Having three fingers on each hand, and two toes on each foot. His slider features consist of light yellow stripe-like markings running down his thighs and arms, sporting two on the sides of his shoulder blades, whilst the other two reside on the sides of his upper thighs. He follows with two red crescent-shaped markings imprinted just on the outer edges of his eyes, having a Goldenrod yellow plastron, as well as a dullish blue grey carapace that has a somewhat darker outer trim around the edges. Plastered on the back is a tri-numbered steel blue pattern that resembles closely to the curly bracket symbol: {}, His eyes are onyx and often rather slanted, probably attributing to his tease-y and 'up to no good' demeanor. Leo wears a Dodger Blue mask that has back-length bandana tails reaching tied at the backside, his forearms donning dark teal fingerless gloves which have a matching dodger blue trim outlined on both sides. The socks on his legs having a matching scheme, reaching just below his knee. The sock lacks coverage on his ankle and toe as well, finishing his attire with a matching blue belt that has two holsters on the back and side, a strap perched on the left of his shoulder. Finally, the turtle emblem is located partially on the right of his plastron.

Señor Hueso is a skeleton Yōkai with a black mustache, dark grey eye sockets, and light grey bones. He wears a dark grey suit paired with a red dress shirt, a black tie, and grey and white spectator shoes. He also wears red socks and a dark grey fedora with a red ribbon. Personality Hueso is serious and responsible. This is shown in his devotion to his restaurant Run of the Mill Pizza, prioritizing its safety even over his own physical health. He isn't the most altruistic person, especially if a situation threatens his serene lifestyle and it can result in stubbornness.

Michelangelo is presented to be a darkish verdant green skinned terrestrial chelonian mutant/yokai who possesses the features of an ornate box turtle, sporting an altered human-like physique consisting of sculpted, yet overall petite figure. Being the shortest among his brothers (both probably due to him being the youngest as well as his miniature turtle species), having three fingers on each hand, and two toes on each foot. His turtle features consist of a bright yellow plastron, a blue grey carapace that has a somewhat darker outer trim around the edges, followed with a bright sunflower yellow pattern displayed on the back. The pattern resembling very closely to the curly bracket symbol {}; Finally, on each side of his shoulder blades, alongside his upper thighs, are matching yellow spots that have a faint orange outline surrounding them. Mikey's eyes are onyx and rather doe, meant to accentuate his role of being the youngest brother. For accessories, Mikey adorns his plastron with two mismatched Robin Egg Blue and magenta colored stickers. One sticker (a triangle) located at the upper left, whilst the other (a lightning bolt) is located at the lower right. Both stickers having the opposite colored trim, he also wears a carrot orange mask that has short, slightly curvy bandana tails tied at the back. Followed by matching orange ankle-length stockings that reveal his ankles and toe, a pair of wristbands, and a pair of knee-pads. Both pads having silly red faces drawn over them. Finally, Mikey sports a Rust brown harness that has the turtle emblem attached over his heart.

Donatello is the Turtle that is commonly accepted as the smartest of the group, specifically in matters related to scientific pursuits and technology. He is an accomplished inventor who is able to develop multiple advanced devices with minimal resources, which he devotes to helping both himself and his brothers. In most incarnations, he wears a purple bandana and wields a Bō staff

Raphael is an alligator snapping turtle who was mutated to become part human. He is one of the main protagonists of Rise of the Teenage Mutant Ninja Turtles. He is the eldest Turtle and the former leader of the Mad Dogs. He wielded a pair of tonfā that generated mystic energy until they were destroyed and replaced by his sai.