Johnny Joestar is a young, well-built man with a strong upper body and was once paralyzed. He has blue eyes, shoulder-length blond hair that curls upward at the ends, and wears a distinctive star-patterned knit cap with a horseshoe ornament on the front, while his riding clothes carry matching star motifs. Johnny is an intensely determined and intelligent person. He becomes more confident through his journey with Gyro. His Stand, Tusk, is a small, strange pink humanoid creature that develops through Acts, beginning with the ability to fire Johnny's fingernails like bullets and eventually reaching the devastating power of infinite rotation.

Gyro Zeppeli is a tall, athletic Italian horseman with long blond hair, sharp features, and a flamboyant riding outfit covered in unusual decorative patterns. Gyro is confident, cocky, sarcastic, competitive, and fond of teasing Johnny, often acting carefree even in dangerous situations. Beneath that humor is a disciplined, compassionate man with a strong sense of duty. His signature weapons are his steel balls, which channel the Spin, a technique based on precise rotation. His ultimate technique manifests Ball Breaker, a tiny hunched humanoid Stand with hoof-like feet, a studded body, and a large spiral-like structure around its head.

Diego Brando is a handsome young British jockey with blond hair, sharp facial features, and an athletic build suited to competitive horse racing. His elaborate riding clothes feature distinctive patterns, emphasizing his flashy, predatory image. Diego is fiercely ambitious, proud, calculating, ruthless, and extremely competitive. He hates losing and constantly searches for advantages, while his intelligence allows him to quickly analyze dangerous situations. Despite his cold nature, he is disciplined and highly capable. His Stand, Scary Monsters, gives him the power to transform into a powerful dinosaur and infect others with the same transformation. In dinosaur form, Diego becomes a terrifying, fast-moving predator with enhanced physical abilities and senses, making him dangerous in close combat and chase.

Lucy Steel is a fourteen-year-old girl of average height and build with blond, medium-length hair and delicate features. She usually wears a distinctive dress with striped tights, arm warmers, and tall rose colored boots, giving her a charming but unusual Victorian-inspired appearance. Despite her young age and innocent appearance, Lucy is intelligent, observant, gentle, courageous, and remarkably resourceful. She can remain calm enough to think clearly while surrounded by people far more powerful than herself. Lucy is especially protective of Steven and repeatedly risks herself to help others. She does not have a Stand, instead relying on her intelligence, courage, and quick thinking to survive.

Steven Steel is a mature, imposing man standing around 190 cm tall, with gray hair styled into a rounded bob-like shape with serrated edges. He wears a high-collared coat decorated with tassels and formal trousers, along with small rectangular glasses that reinforce his refined appearance. As the organizer and public face of the Steel Ball Run, Steven presents himself as bold, authoritative, eccentric, and confident, speaking dramatically and handling problems with apparent composure. Privately, however, he is much more anxious and uncertain, carrying doubts beneath his theatrical personality. He dreams less of enormous fame than simply having his contribution recognized. Steven has no Stand and is not a frontline fighter, but his intelligence, determination, and devotion to Lucy make him an important figure.

Hot Pants is a mysterious young woman who disguises herself as a man. Her short, pink-colored hair, riding clothes, gloves, boots, give her a rugged appearance that makes her difficult to read. She is serious, guarded, pragmatic, and secretive, rarely exposing her emotions or true motives. Hot Pants is willing to be ruthless when necessary, but she has a strong compassionate side and repeatedly helps people. Her Stand, Cream Starter, takes the unusual form of a spray-can-like tool that produces living flesh. The substance can be molded to disguise appearances, cover or restrain people, damage enemies, and even heal injuries, making the Stand extraordinarily versatile.

Pocoloco is a Black horse racer with an average-to-tall height, medium build, dark hair, and a thin horseshoe-shaped mustache. He wears light-colored clothing and a distinctive headpiece resembling an old-fashioned leather aviator helmet covered with oval decorations, giving him a cheerful and eccentric appearance. Pocoloco is extraordinarily carefree, optimistic, friendly, and confident, often treating incredible dangers as though everything will naturally work out. After learning that he supposedly possesses extraordinary luck, his confidence becomes almost ridiculous. His Stand, Hey Ya!, is a tiny humanoid figure with a bucket-like head, uneven eyeholes, sharp little teeth, bolts, thin limbs, shoulder pads, and rope-like strands around its head. Rather than fighting, it constantly encourages and advises Pocoloco, helping him make the most of his unbelievable luck.