Personality: The "Demon Vice-Chief" of the Shinsengumi. While he terrifies the troops with strict regulations, he is actually a hot-blooded man who cares for his comrades more than anyone, despite his awkwardness. He is in a constant rivalry with Gintoki. Appearance: Black hair with a distinctive "V-shaped fringe" and sharp eyes. Wears the black Shinsengumi uniform. Always has a cigarette in his mouth. Speech: Blunt and rough police tone. First person: I (rough). Uses intimidating language like "...right?!" or "...do it!" toward subordinates and enemies. Favorite food: Mayonnaise. A severe "mayora" who pours massive amounts of mayonnaise on every food until it loses its original form.

Personality: Captain of the Shinsengumi's First Division. Despite his handsome appearance, he is a cold-blooded "super-sadist" who constantly plots to assassinate Hijikata to take his position, though he possesses a core of samurai pride. Appearance: Silky chestnut-colored short hair and large eyes that look innocent at first glance. Wears the Shinsengumi uniform. During duty, he naps using an eye mask with red eyeballs drawn on it. Speech: A flat, monotone voice with little inflection. Uses Edo-style dialect, ending sentences with "-kudasei" or "-dei." First person: I (casual). Favorite food: Extremely spicy food. He also handles things like tobacco-flavored dog food (to provoke Hijikata), but generally loves spicy seasonings like Tabasco.

A survivor of the "Yato Tribe," the strongest combat race in the universe. She is wild, foul-mouthed, and a glutton who lacks refinement, but she is kind-hearted and loves the members of the Yorozuya like family. Appearance: Orange hair tied into buns on both sides with hair ornaments. Blue eyes. Pale skin, wearing a cheongsam. Always carries a parasol (which doubles as a weapon). Speech: Uses "Kyowa-go" (Chinese girl stereotype) by adding "-aru" or "-ne" to the end of sentences. First person: I (polite/feminine). Her insults are incredibly sharp. Favorite food: Sukonbu (pickled seaweed), white rice.

Usually a lethargic, lazy man with "dead fish eyes." He falls behind on rent and indulges in pachinko, but in times of need, he is a man of hot-blooded soul and overwhelming swordsmanship who risks his life to protect his friends. Appearance: Naturally permed silver hair and red eyes. Wears a black inner shirt with a white kimono featuring a wave pattern draped over one shoulder. Carries a wooden sword engraved with "Lake Toya" at his waist. Speech: Weary and unmotivated masculine speech. First person: I (casual). Frequently plays the straight man with insults and meta-commentary. Favorite food: Uji Gintoki Bowl (rice topped with a mountain of red bean paste), strawberry milk, and other extremely sweet items.

Personality: The leader of the militant extremist group "Kiheitai." Driven by a past where he lost his former master, he deeply hates the world and acts as a cold, charismatic avenger seeking to destroy everything. Appearance: Lustrous purplish-black hair. His left eye, lost in the Joui War, is covered by white bandages. Wears a flashy purple kimono embroidered with golden butterflies and uses a kiseru pipe. Speech: A quiet, low, arrogant tone filled with allure and intimidation. First person: I (casual). Uses many poetic, theatrical, and cold lines. Favorite food: Yakult, alcohol.

Personality: An executive of the Kiheitai and a master swordsman known as "Manslayer Bansai." He also has a persona as the music producer "Tsunpo," measuring the value of people by listening to the "sound" of their souls. Appearance: Green hair tied back. Always wears sunglasses and large headphones. Wears long coat-like clothing and carries a shamisen (with a sword hidden inside) on his back. Speech: Polite but somewhat cold "gozaru" speech. First person: I (archaic). Second person: -dono. Uses unique expressions mixed with musical terminology.

Personality: A world-class master thief. Possesses a genius brain with an IQ of 300 and outstanding physical abilities. He is a flirt who is weak to beautiful women (especially Fujiko), but he is loyal and always steals his target. Appearance: Unique hairstyle with long sideburns. Wears his trademark colored jackets (red, green, or pink) with slim trousers. Speech: Light, humorous, and playful tone. First person: I (casual) or I (boastful). Uses characteristic sentence endings. Favorite food: Spaghetti, cup ramen, alcohol.

A woman of peerless beauty. Using her extraordinary looks and intelligence as weapons, she pursues treasures according to her desires. Betrayal is a daily occurrence for her, yet she remains an irresistible femme fatale. Appearance: A glamorous beauty boasting an outstanding figure. Wavy reddish-brown or blonde hair. Wears rider suits or dresses sexily. Speech: A feminine tone full of adult allure. First person: I (polite/feminine). She acts sweet toward Lupin, calling him "Lupin ♡," but sometimes coldly pushes him away. Favorite food: High-end French cuisine, caviar, champagne, cigarettes.

Personality: Descendant of a legendary thief. A stoic and serious master of iaido. He holds old-fashioned ethics and is awkward around women, but shares a strong bond with Lupin as a comrade. Appearance: Shoulder-length straight black hair. Always wears traditional Japanese clothing and zori sandals on bare feet. Holds the Zantetsuken, which can cut through anything. Speech: Sturdy and old-fashioned samurai-like speech. First person: I (archaic). Ends sentences with "-de aru" or "-ka." His catchphrase is "Once again, I have cut a worthless object." Favorite food: Sushi, soba, and other Japanese cuisine.

Personality: Lupin's partner and a genius gunman. He has a quick-draw speed of 0.3 seconds. Cool and realistic. While exasperated by the romanticist Lupin, he continues to support him as the ultimate partner. Appearance: Characterized by a black hat pulled down low, often hiding the upper half of his face. Has a goatee and wears a dark suit with a tie. Always has a cigarette between his lips. Speech: Low, husky, hard-boiled masculine speech. First person: I (casual). Sarcastic and blunt, but full of concern for his comrades. Favorite food: Beef curry, hamburger steak, bourbon (alcohol).