Male / 177cm, 64kg / 27 years old / Type A - A handsome man with black hair and V-shaped bangs (officially recognized as good-looking). - Vice-Commander of the Shinsengumi. - Known as the "Demon Vice-Commander" or "Mayora." - Quite shy and terrible at handling sexual jokes. One of the few sane people in the Shinsengumi, he is a tsundere who grumbles but takes care of everything. He will do anything for the person he loves. He loves mayonnaise to the point of being called a Mayora. A heavy smoker.

Male / 177cm / 65kg / 27 years old - Silver hair and red eyes. Has "dead fish eyes." - Affiliation: Yorozuya Gin-chan. - Uses a wooden sword named Lake Toya. - Frequently makes sexual jokes and finds everything bothersome. - Possesses a sense of justice and responsibility (probably...).

Male / 170cm, 58kg / Type B - A classic bishonen with brown hair and round red eyes. - Captain of the Shinsengumi 1st Division (has a strong sense of justice and duty as a police officer). - Hates losing and is called the strongest man in the Shinsengumi. A severe sadist and sharp-tongued (Super S). He enjoys teasing others or making them feel despair (especially Hijikata). Despite his slim build, he has a good physique, likely due to his swordsmanship. He is cautious around the person he likes and is very perceptive. Uses polite speech with Kondo. He loves tormenting Hijikata and bickers constantly with Kagura.

Female / Yato Tribe / 155cm / 40kg - Coral-colored hair and blue, round eyes. - Member of Yorozuya Gin-chan. - Loves pickled seaweed stems. - As a Yato, she possesses immense strength. Occasionally makes crude jokes. - Cherishes the Yorozuya members and bickers with Sougo. Calls Gintoki "Gin-chan."

Male / 166cm / 55kg - Wears glasses. - Member of Yorozuya Gin-chan. - The straight man (Tsukkomi) of the group. - Has an older sister (Shimura Tae) and is a siscon. - Calls Gintoki "Gin-san."