A female hero with the face of a leopard, wearing a hero suit with a cape. 25 years old. Her true name is Sena Hyodo. She is an old acquaintance of Lionheart from his time in Africa, reuniting after 15 years. She is the daughter of a doctor in the village where Lionheart used to volunteer. After Lionheart left Africa, she assisted her father while aspiring to become a doctor herself. One day, she discovered that the poaching syndicate Lionheart was supposed to have dismantled had returned. While fleeing from them, a ring she had bought at a night market began to glow, transforming her into Panthera. Upon learning that Kingsley, the boss of the poaching syndicate Lionheart once defeated, is still alive, she travels to Japan. Normally, she is a cheerful woman with bright hair. Having only recently become a hero, she tends to be hot-headed. Powers: Moves at speeds invisible to the human eye and leaps around freely. While not as strong as Lionheart, she possesses significant power and sharp claws. Unlike Lionheart, she can also transform completely into a leopard, at which point her speed becomes several times faster than her beast-man form.

A man with the face of a black lion, a muscular build, and a hero suit themed in blue and black. Lionheart's rival. Like Lionheart, he transforms into Dark Lion using one of the Artifacts of the Earth. While he shares the mission of being a guardian of nature and life, he harbors a deep loathing for humanity and does not hesitate to kill if necessary. He possesses abilities nearly identical to Lionheart's. In the past, he clashed with Lionheart in Africa after destroying a factory that was dumping toxic waste and attempting to slaughter everyone inside. After losing that battle, he was missing for a long time, but his survival was recently confirmed abroad. His true name is Takashi Kuroe. 40 years old. Formerly a zoologist. On the surface, he is a calm and intellectual individual, but inwardly he harbors a burning rage toward humans who destroy nature and drive countless species to extinction without a second thought. When he transforms into Dark Lion, his speech becomes rough and aggressive, exploding with inner rage to cause utter destruction.

The boss of the poaching syndicate that once shot Go Shishihara (Lionheart). Male. 62 years old. A tall man who wears expensive suits and appears to be a perfect gentleman at first glance. He wears a monocle and is of British aristocratic descent. He always speaks with a polite tone, though his arrogance is palpable. He has a morbid obsession with hunting and taxidermying various wild beasts, believing that "because they are strong and noble, they deserve to be preserved as specimens." He is a billionaire who owns multiple corporations and is a master marksman himself. Although his organization was dismantled by Lionheart and he was presumed missing, he has been hiding underground, waiting for his moment. His current ultimate goal is to hunt and taxidermy the strongest and most beautiful beast in the world: Lionheart.

Male. 40 years old. A scientist who looks like a chimpanzee and wears a lab coat. He was once an unremarkable scientist working for one of Kingsley's companies. He performed experiments to increase his own intelligence; while his intellect improved, his body regressed into a simian state. He currently develops gadgets for Kingsley. Due to the backlash of being looked down upon in the past, he has become extremely arrogant, yet he remains pathetically submissive and unable to defy Kingsley.

Female. 33 years old. A clerk at "Savanna Express," the company where Lionheart works in his human form. Since it is a small company, she handles many roles, and even the president can't stand up to her. She has a favorable impression of Go Shishihara (Lionheart), but she is frustrated by the fact that despite being a diligent worker, he frequently disappears without notice.