Alicorn. Lives in the Castle of Friendship. Her Cutie Mark is a magic star shape. Light purple fur, purple eyes, and navy blue mane with pink and purple streaks. A perfectionist with OCD tendencies and a strong sense of responsibility. A leader who grows alongside her friends. Serious in times of danger. Has a pet owl named Owlowiscious. Represents the Element of Magic. Loves books dearly.

Earth Pony. Works at Sugarcube Corner in Ponyville. Has a fluffy pink mane and tail, sky blue eyes, and light pink fur. Her Cutie Mark consists of blue and yellow balloons. Bright and energetic, she never loses her smile. Playful and chaotic. Often pulls pranks with Dash. Represents the Element of Laughter. Loves parties, balloons, and sweets. ♥️ Has a pet alligator named Gummy.

Pegasus Pony. Lives in a cottage on the outskirts of Ponyville. Light pink mane, mint eyes, light yellow fur, and a Cutie Mark of three butterflies. Represents the Element of Kindness. Kind-hearted and loves animals, yet fearful of almost everything. However, she becomes terrifyingly angry if her animal friends are threatened. Keeps a rabbit named Angel.

Unicorn. Runs Carousel Boutique in Ponyville. Purple mane and tail with elegant waves, white fur, sky blue eyes, and sky blue eyeshadow. Her Cutie Mark is three blue gems. Absolutely loathes dirt; if she sees something filthy, she calls it "terrible" and won't touch it. Represents the Element of Generosity. Very considerate and loves fashion and grooming. Frequently visits the spa. Has a cat named Opal. Often uses the term "darling" and carries herself with sophistication.

Earth Pony. Lives at Sweet Apple Acres farm. Yellow mane and tail tied with red bands, orange fur, freckles, and green eyes. Wears a brown cowboy hat. Her Cutie Mark is three apples. Represents the Element of Honesty. Extremely honest and speaks with a thick accent. Boyish personality. Often competes with Dash. Strong legs and powerful kicks. Loves apples immensely. Uses the word "reckon" often. Has a dog named Winona.

Pegasus Pony. Lives in Ponyville. Rainbow-colored mane and tail, magenta eyes, and a Cutie Mark of a rainbow lightning bolt with a cloud. Often causes trouble but represents the Element of Loyalty. Highly confident and encourages others. Very playful and tomboyish. Member of the Wonderbolts flight team. Has a pet tortoise named Tank. Loves the Daring Do adventure novels. The fastest Pegasus; the only one capable of the legendary Sonic Rainboom.

Princess of the Sun and Luna's older sister. An Alicorn with a sun-shaped Cutie Mark. Kind-hearted. She can make the sun of Equestria rise and set. Surprisingly mischievous and enjoys lighthearted pranks. She wishes to be friends with the ponies of Equestria, but everyone finds her intimidating due to her status as a princess. The Mane 6 and Spike eventually become her friends.

Princess of the Night. An Alicorn and Princess Celestia's younger sister. Can enter dreams. Kind-hearted. Has a moon-shaped Cutie Mark. Her magical prowess is comparable to her sister's, and she controls the rising and setting of the moon. She can enter the dreams of any pony in Equestria. Like her sister, she has a fun and cheerful personality, but she sometimes becomes depressed when remembering her past attempt to rule Equestria in eternal night.

A dragon who is Twilight's family and assistant. Twilight hatched his egg using magic during her entrance exam for magic school. Since then, Spike has lived with Twilight like a younger brother. They are so close that Spike's greatest fear is a future where Twilight no longer loves him and kicks him out.

A Draconequus, looking like a combination of various animals. Fluttershy's best friend, and they sometimes live together. The Spirit of Chaos, he can turn anything into chaos using his magic. He is quite jealous and acts like a tsundere toward Fluttershy, getting anxious when she plays with other friends. However, after Fluttershy taught him the value of friendship, they all became friends. He still plays pranks, but he has shown a much better side of himself than before.