Ezio Auditore da Firenze, A Florentine Assassin with dark brown hair, short stubble, sharp features, and the distinctive scar across his mouth. He wears his white and red Assassin robes with the armor and equipment of his Brotherhood era appearance. Ezio is charismatic, confident, witty, observant, protective, charming, and highly skilled. After the destruction of Monteriggioni and Mario’s death, he is in Rome trying to rebuild the assassins and take revenge on the Borgia

Claudia Auditore da Firenze — Ezio’s younger sister, with medium length dark brown hair and refined features. She wears elegant Renaissance dresses and carries herself with increasing confidence. Claudia is intelligent, organized, outspoken, stubborn, and fiercely protective of her family. She runs the Rosa in Fiore and becomes increasingly involved with the Assassins. Maria Auditore da Firenze — Ezio and Claudia’s mother, a middle-aged noblewoman with long dark hair and refined features. She dresses in elegant Renaissance clothing appropriate to her background. Maria is gentle, cultured, intelligent, and deeply protective of her children. After the destruction of Monteriggioni, she helps Claudia with the Rosa in Fiore. Leonardo da Vinci — A middle-aged man with shoulder length dark blonde hair beginning to gray, a full beard, and the appearance of an eccentric Renaissance scholar. He usually wears simple period clothing. He is brilliant, curious, friendly, eccentric, and fascinated by science, art, engineering, and invention. He is one of Ezio’s closest friends and creates and repairs many of Ezio’s tools and equipment. Niccolò Machiavelli — A dark-haired, bearded man dressed in dark blue Renaissance clothing. He is intelligent, calculating, pragmatic, blunt, and highly strategic. He is a senior member of the Italian Assassin Brotherhood and becomes one of Ezio’s most important advisers after Mario’s death. He often favors careful planning and practical results over Ezio’s more idealistic approach. La Volpe — The mysterious leader of the Roman Thieves’ Guild and a member of the Italian Assassin Brotherhood. He has dark hair and a weathered appearance and wears the distinctive hooded clothing associated with his role as a thief. He is clever, secretive, resourceful, suspicious, and sarcastic. After the fall of Monteriggioni, he moves to Rome and fights against Borgia influence. Bartolomeo d’Alviano — A large, imposing Italian condottiero and the leader of the Roman mercenaries. He has a muscular build, dark hair, and a strong, battle-hardened appearance, usually wearing heavy military clothing and armor. Bartolomeo, fearless, passionate, loyal, and extremely confident, with a great love of fighting and military honor. He is a member of the Assassin Brotherhood and becomes one of Ezio’s major allies in Rome. Cesare Borgia — A tall, imposing man with long dark hair, a strong jaw, and a neatly trimmed beard. He wears elaborate black, red, and military clothing befitting his position. Cesare is ambitious, arrogant, aggressive, charismatic, ruthless, and obsessed with power. He commands the Papal Army and personally led the attack on Monteriggioni, where he killed Mario and seized the Apple of Eden. Lucrezia Borgia — A young noblewoman with long blonde hair, pale skin, and refined features. She wears elaborate and luxurious Renaissance gowns befitting the Borgia family. Lucrezia is intelligent, manipulative, spoiled, politically influential, and accustomed to luxury and power. She is Rodrigo’s daughter and Cesare’s sister and is deeply entangled in the Borgia family’s political affairs. Rodrigo Borgia — An older man with gray hair, a broad face, and a carefully groomed beard. As Pope, he wears elaborate white and red papal robes and carries himself with considerable authority. Rodrigo is intelligent, calculating, politically powerful, cautious, and manipulative. He is the father of Cesare and Lucrezia, the head of the Borgia family, and the Grand Master of the Templars.